|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||1 µg/ml|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||10µg/ml|
|Immunoprecipitation (IP)||Assay Dependent|
|Radioimmune Assays (RIA)||Assay Dependent|
|Western Blot (WB)||2 - 4 µg/ml|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Horse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified human beta-2 Microglobulin.|
|Purification||Ammonium sulfate precipitation, Caprylic acid precipitation|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody reacts with beta2-microglobulin associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. It will not cross-react with mouse, rabbit, canine, chicken or bovine.
beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B2M; Beta 2 microglobulin precursor; Beta chain of mhc class 1 proteins; beta chain of MHC class I molecules; beta-2-microglobin; Beta-2-microglobulin; Hdcma22p; Orla-B2m
Gene Aliases: B2M; CDABP0092; HDCMA22P; IMD43
UniProt ID: (Human) P61769
Entrez Gene ID: (Human) 567
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