|Immunohistochemistry (Paraffin) (IHC (P))||1:250-1:500|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide from the C-terminus of the beta-catenin protein|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C|
This antibody reacts with the C-terminal portion of beta-Catenin.
For staining of formalin-fixed, paraffin-embedded tissues, it is recommended to boil tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at room temperature for 20 min. Recommended positive controls are MCF-7 cells and breast carcinoma tissue.
This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
The catenins (alpha, beta and gamma) are ubiquitously expressed, cytoplasmic proteins associated with E-cadherin at cellular junctions. beta-catenin also binds to N-cadherin and co-immunoprecipitates with APC. Cadherin/catenin complexes are linked to the cytoskeleton via a direct association between alpha-actinin and alpha-catenin. Increases tyrosine phosphorylation can disrupt catenin-cadherin complexes, influencing cellular adhesion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Beta-catenin; catenin; catenin (cadherin-associated protein), beta 1; catenin (cadherin-associated protein), beta 1, 88kDa; Catenin beta; Catenin beta-1; CATNB; CTNB1; CTNNB
Gene Aliases: armadillo; Bfc; Catnb; CTNNB; CTNNB1; Mesc; MRD19; OK/SW-cl.35; PRO2286
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.