|Flow Cytometry (Flow)||2 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
|Western Blot (WB)||1:50-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Dog, Human, Rat|
|Published species reactivity||Pig Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide from the cytoplasmic domain of human CD117 / c-kit protein|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C|
PA5-16770 targets CD117 in WB, IHC (P), FACS and ICC/IF applications and shows reactivity with Canine, Rat and Human samples.
The PA5-16770 immunogen is a synthetic peptide from the cytoplasmic domain of human CD117 / c-kit protein.
Recognizes a protein of 145kDa, which is identified as CD117/p145kit. This rabbit polyclonal antibody does not interfere with the binding of SCF to c-kit. It precipitates both the unoccupied as well as the occupied form of c-kit. The binding of the stem cell factor (SCF) to the c-kit-encoded receptor tyrosine kinase (Type III) stimulates a variety of biochemical responses that culminate in cellular proliferation, migration, or survival. C-kit plays an important role in hematopoiesis, melanogenesis, and gametogenesis. This antibody is excellent for staining of formalin-fixed, paraffin-embedded tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: C-Kit; c-KIT gene1; CD117; ckit; Mast/stem cell growth factor receptor Kit; p145 c-kit; PBT; Piebald trait protein; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; receptor tyrosine kinase c-kit; SCFR; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
Gene Aliases: C-Kit; CD117; KIT; PBT; SCFR
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