|Flow Cytometry (Flow)||1:20|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
|Western Blot (WB)||1:500-1:1000|
|Western Blot (WB)||See 6 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Rat , Non-human primate , Mouse , Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human eIF-2-alpha.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: EIF-2; eIF-2-alpha; EIF-2A; EIF-2alpha; EIF2; EIF2A; EIF2S1; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; eukaryotic translation initiation factor 2A
Gene Aliases: 0910001O23Rik; 2410026C18Rik; 35kDa; EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A; eIF2alpha; EIF2S1
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