|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||0.2 µg/ml|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified, His-tagged, recombinant, human gamma enolase protein expressed in E. coli.|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
A suggested positive control for this product is SH-SY5Y, U87mg or mouse brain.
Enolase (2-phosphoglycerate hydrolyase or phosphopyruvate hydrates) is a glycolytic enzyme that catalyzes the dehydration and conversion of 2-phosphoglycerate to phosphoenolpyruvate. It comprises three distint subunits, alpha, beta and gamma. The gamma/gamma and alpha/gamma dimeric forms of enolase, referred to as neuronspecific enolase (NSE), are localized mainly in neurons and neuroectodermal tissue. NSE has a high stability in biological fluids and can easily diffuse to the extracellular medium and cerebrospinal fluid (CSF) when neuronal membranes are injured. NSE is used clinically as a sensitive and useful marker of neuronal damage in several neurological disorders including stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 2-phospho-D-glycerate hydro-lyase; 2-phospho-D-glycerate hydrolyase; Enolase 2; enolase 2 (gamma, neuronal); epididymis secretory protein Li 279; Gamma-enolase; Neural enolase; neuron specific gamma enolase; Neuron-specific enolase; neuronal enriched enolase; neurone-specific enolase; NSE
Gene Aliases: AI837106; D6Ertd375e; Eno-2; ENO2; HEL-S-279; NSE
Molecular Function: lyase
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