|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 293 and 528 of Human hnRNP 1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-27429 targets hnRNP 1 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-27429 immunogen is recombinant fragment corresponding to a region within amino acids 293 and 528 of Human hnRNP 1.
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.
Protein Aliases: Helix-destabilizing protein; Heterogeneous nuclear ribonucleoprotein A1; heterogeneous nuclear ribonucleoprotein A1B protein; heterogeneous nuclear ribonucleoprotein B2 protein; heterogeneous nuclear ribonucleoprotein core protein A1; heterogeneous nuclear ribonucleoprotein I; heterogeneous nuclear ribonucleoprotein polypeptide I; hnRNP A1; hnRNP core protein A1; hnRNP core protein A1-like 3; HNRNP-I; HNRNPI; HNRPI; nuclear ribonucleoprotein particle A1 protein; polypyrimidine t; polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I); pPTB; PTB-1; PTB-T; PTB2; PTB3; PTB4; putative heterogeneous nuclear ribonucleoprotein A1-like 3; RNA-binding protein; single-strand DNA-binding protein UP1; Single-strand RNA-binding protein
Gene Aliases: ALS19; ALS20; hnRNP A1; hnRNP-A1; HNRNPA1; HNRPA1; HNRPA1L3; IBMPFD3; UP 1
UniProt ID: (Human) P09651
Entrez Gene ID: (Human) 3178