|Western Blot (WB)||1:200 - 1:800|
|Immunohistochemistry (Paraffin) (IHC (P))||See 2 publications below|
|Western Blot (WB)||See 35 publications below|
|Immunocytochemistry (ICC)||See 4 publications below|
|Immunohistochemistry (IHC)||See 47 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Avian , Rabbit , Rat , Pig , Amphibian , Cat , Fish , Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues D(17) L K E E K D I N N N V K K T(31) of mouse iNOS.|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-036 detects inducible nitric oxide synthase (iNOS) from human, mouse and rat tissues and cells as well as recombinant human and mouse iNOS. This antibody does not detect other NOS isoforms.
PA1-036 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~135 kDa protein representing mouse iNOS from LPS stimulated RAW 264.7 cells.
The PA1-036 immunogen is a synthetic peptide corresponding to residues D(17) L K E E K D I N N N V K K T(31) of mouse iNOS.
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.
iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: HEP-NOS; Hepatocyte NOS; Inducible Nitric Oxide Synthase; Inducible NO synthase; Inducible NOS; MAC-NOS; Macrophage NOS; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); Nitric oxide synthase, inducible; nitric oxide synthase, macrophage; NOS Type II; NOS, type II; Peptidyl-cysteine S-nitrosylase NOS2
Gene Aliases: HEP-NOS; i-NOS; INOS; Inosl; NOS; Nos-2; NOS-II; NOS2; NOS2A
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