|Immunohistochemistry (Paraffin) (IHC (P))||Ready-to-use|
|Immunocytochemistry (ICC)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Rat Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of mouse i-NOS|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is lung tissue.
NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a mesenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. Inducible NOS (iNOS) or macrophage NOS (mNOS) is a calcium/calmodulin independent and is expressed in activated macrophages and stimulated glial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: HEP-NOS; Hepatocyte NOS; Inducible Nitric Oxide Synthase; Inducible NO synthase; Inducible NOS; nitric oxide synthase 2, inducible; nitric oxide synthase 2A (inducible, hepatocytes); Nitric oxide synthase, inducible; nitric oxide synthase, macrophage; NOS Type II; NOS, type II; Peptidyl-cysteine S-nitrosylase NOS2
Gene Aliases: HEP-NOS; INOS; NOS; NOS2; NOS2A
UniProt ID: (Human) P35228
Entrez Gene ID: (Human) 4843
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