|Western Blot (WB)||1:2500-1:5000|
|Tested Species reactivity||Human, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to amino acids 2447-2549 of human mTOR expressed in E. coli.|
|Storage buffer||PBS with 30% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
Western blot analysis of PA1-188 detects mTOR at ~250 kDa in human and non-human primate cells, slightly below the theoretical MW of ~280kDa. Using a Tris-Acetate SDS gel for high molecular weight protein separation, and a nitrocellulose membrane for protein transfer are necessary for optimal WB results.
Immunofluorescence analysis shows cytoplasmic and some nuclear staining of mTOR in human B3 and U-2 OS cells.
Mechanistic target of rapamycin (mTOR) is a serine/threonine protein kinase which is a central regulator of cellular metabolism, growth, and survival in response to hormones, growth factors, nutrients, energy, and stress signals. mTOR directly or indirectly regulates the phosphorylation of at least 800 proteins, and functions as part of 2 structurally and functionally distinct signaling complexes, mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. As part of the mTORC2 complex, mTOR regulates cellular processes including survival and organization of the cytoskeleton. It plays a critical role in the phosphorylation at Ser-473 of AKT1. Mechanistic target of rapamycin complex 1 (mTORC1) contains mTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC2 contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR. Due to its central role in celliular signaling, mTOR is associated with a vast array of diseases, including many cancers, inflammation, cellular growth, and metabolic disorders.
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Protein Aliases: EC 18.104.22.168; FK506 binding protein 12-rapamycin associated protein; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP-Rapamycin Associated Protein; FKBP12-rapamycin complex-associated protein; FKBP12-rapamycin complex-associated protein 1; FRAP1; FRAP2; m-TOR; Mammalian Target Of Rapamycin; Mechanistic Target Of Rapamycin; mechanistic target of rapamycin (serine/threonine kinase); MTOR; mTORC1; RAFT1; Rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; Rapamycin Target Protein; Rapamycin target protein 1; Serine/Threonine-Protein Kinase MTOR
Gene Aliases: FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; SKS
UniProt ID: (Human) P42345
Entrez Gene ID: (Human) 2475
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