|Immunohistochemistry (Frozen) (IHC (F))||1:800|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-400|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Western Blot (WB)||See 3 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Bovine, Human, Mouse, Rabbit, Rat|
|Published species reactivity||Rat , Zebrafish , Mouse , Human , Guinea pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues T(724) K R R A I G F K K L A E A V K(739) C of rat bNOS.|
|Storage buffer||whole serum diluted in PBS|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA3-032A detects brain nitric oxide synthase (bNOS) from bovine, mouse, rabbit and rat tissues. This antibody does not detect endothelial NOS (eNOS) or inducible NOS (iNOS).
PA3-032A has been successfully used in Western blot, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects an ~155 kDa protein representing bNOS from rat brain extract. Immunohistochemical staining of bNOS in rat brain with PA3-032A results in staining of the anterior pituitary.
The PA3-032A immunogen is a synthetic peptide corresponding to residues T(724) K R R A I G F K K L A E A V K(739) C of rat nNOS.
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.
eNOS and bNOS share ~50% sequence homology, and their activity depends on binding to the calcium/calmodulin complex. Both constitutive isoforms respond immediately to increased levels of calcium to produce low levels of NO over a short period of time. bNOS has been found in neurons, peripheral nerve cells, macula densa and pancreatic islet cells.
Protein Aliases: BNOS; bNOS1; Brain NOS; Constitutive NOS; N-NOS; NC-NOS; Neuronal Nitric Oxide Synthase; Neuronal NOS; nitric oxidase synthase; nitric oxide synthase 1 (neuronal); Nitric oxide synthase, brain; nNOS; NOS Type 1; NOS type I; Peptidyl-cysteine S-nitrosylase NOS1
Gene Aliases: 2310005C01Rik; Bnos; IHPS1; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; NOS-I; NOS1