|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2-3 µg/mL|
|Immunofluorescence (IF)||2-3 µg/mL|
|Western Blot (WB)||1:500|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) N F L R G V M G G Q S A G P Q H(17) of rat p115.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-006 detects p115 in rat and mouse samples.
PA1-006 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~115 kDa protein representing p115 in rat and mouse liver samples.
The PA1-006 immunogen is a synthetic peptide corresponding to residues M(1) N F L R G V M G G Q S A G P Q H(17) of rat p115. This sequence is conserved across many species including mouse and human. PA1-006 immunizing peptide (Cat. # PEP-276) is available for use in neutralization and control experiments.
p115 (Vesicle docking protein p115) is a peripheral membrane protein that is located on the Golgi complex. p115 exists as a homodimer with two globular heads, an extended coiled-coil tail, followed by an acidic domain at the extreme C terminus. p115 is homologous to a yeast protein, Uso1p, which is required for ER to Golgi transport. p115 likely plays an important role in vesicle transportation from the ER to the cis-Golgi comparments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: General vesicular transport factor p115; Protein USO1 homolog; TAP; Transcytosis associated protein; transcytosis associated protein p115; Transcytosis-associated protein; USO1 homolog, vesicle docking protein; USO1 vesicle docking protein homolog; Vdp; Vesicle docking protein ; vesicle docking protein p115; vesicle docking protein, 115 kDa; Vesicle-docking protein
Gene Aliases: 115kDa; P115; TAP; USO1; VDP
Molecular Function: membrane traffic protein
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