|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Western Blot (WB)||See 2 publications below|
|Tested Species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||A synthetic peptide from p130 corresponding to aa878-913|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-14305 targets p130/Rb2 in IF, IHC (P), IP, and WB applications and shows reactivity with Human and Rat samples.
The MA5-14305 immunogen is a synthetic peptide from p130 corresponding to aa878-913.
Two retinoblastoma-related proteins, p107 and pRb2/p130 are structurally and functionally similar to the product of the retinoblastoma gene (pRb/p105). Like pRb they bind transforming proteins of DNA tumor viruses through a particular region called the "pocket domain". Both proteins play a fundamental role in growth control. The highest percentage of undetectable levels and the tightest inverse correlation with the histological grading and with PCNA expression in the most aggressive tumor types were found for pRb2/p130, which may suggest an important role for this protein in the pathogenesis and progression of lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 130 kDa retinoblastoma-associated protein; P130; PPAR-alpha-interacting complex protein 128; PRB2; PRIC128; RBR-2; retinoblastoma-like 2; Retinoblastoma-like protein 2; Retinoblastoma-related protein 2
Gene Aliases: P130; RB2; RBL2
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