|ChIP assay (ChIP)||1-3 ul|
|Western Blot (WB)||1-2 µg/ml|
|ChIP assay (ChIP)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues G(139) T S G P N Q G P T Q S T(151) C in the nuclear factor binding domain of human p300.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-848 detects p300 from human cells.
PA1-848 has been successfully used in Western blot and ChIP procedures. By Western blot, this antibody detects an ~265 kDa protein representing p300 from HeLa cell lysate.
The PA1-848 immunogen is a synthetic peptide corresponding to residues G(139) T S G P N Q G P T Q S T(151) C in the nuclear factor binding domain of human p300. The PA1-848 immunizing peptide (Cat. # PEP-053) is available for use in neutralization and control experiments.
Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators and have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB, c-Fos, c-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD. Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes. As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription.
Single point mutations in CBP have been proposed as causative factors in the developmental abnormalities of Rubinstein-Taybi syndrome (RTS). Although both CBP and p300 appear to function similarly, the inability of p300 to rescue CBP malfunction in RTS suggests intrinsic functional differences between CBP and p300.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: E1A-associated protein p300; E1A-binding protein, 300kD; EP300; Histone acetyltransferase p300; p300 HAT
Gene Aliases: EP300; KAT3B; P300; RSTS2
UniProt ID: (Human) Q09472
Entrez Gene ID: (Human) 2033
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