|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:200|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the carboxy terminal region of mouse p38.|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
OPA1-10080 detects p38 from human, mouse and rat samples.
OPA1-10080 has been successfully used in Western blot, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~38 kDa protein representing p38 from HeLa whole cell lysates.
OPA1-10080 immunizing peptide corresponds to the carboxy terminal region of mouse p38.
The mitogen-activated protein kinases (MAPK) consist of several subgroups, including the ERK, JNK, and p38 kinases. The members of this MAPK family are regulated by many different extracellular cues ranging from cytokines, growth factors, and neuropeptides. These stimuli activate cell surface receptors to stresses such as cold, heat, osmolarity changes and irradiation. The pathways regulated by the MAPKs control a broad array of cellular responses ranging from survival, cell proliferation, and apoptosis. The MAPKs family is also characterized by their requirement for dual phosphorylation at a conserved threonine and tyrosine residue for enzymatic activation and both must be phosphorylated for full enzymatic activation. The closely related ERK1 (44 kDa) and ERK2 (42 kDa) kinases are characterized by their requirement for dual phosphorylation at a conserved T-E-Y motif. While JNK1 is activated by dual phosphorylation at a T-P-Y motif and p38 is also activated by dual phosphorylation at a T-G-Y motif.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CRK1; CSAID-binding protein; Csaids binding protein; CSBP1; cytokine suppressive anti-inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug binding protein 1; Cytokine suppressive anti-inflammatory drug-binding protein; cytokine-supressive anti-inflammatory drug binding protein; MAP kinase 14; MAP kinase 2; MAP kinase MXI2; MAP kinase p38 alpha; MAPK 14; MAPK14; MAX-interacting protein 2; mitogen activated protein kinase 14; Mitogen-activated protein kinase 14; mitogen-activated protein kinase 14A; Mitogen-activated protein kinase p38 alpha; p38 alpha; p38 MAP kinase; p38 MAP kinase alpha; p38 MAPK; p38 mitogen activated protein kinase; p38-Alpha; p38alpha Exip; p38MAPK; reactive kinase; SAPK2A; Stress-activated protein kinase 2a; tRNA synthetase cofactor p38
Gene Aliases: CRK1; CSBP; CSBP1; CSBP2; CSPB1; EXIP; Hog; MAPK14; MXI2; p38; p38-alpha; p38a; p38ALPHA; p38Hog; p38MAPK; PRKM14; PRKM15; RK; SAPK2A
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