|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of human ERK-1 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody is predicted to react with canine and mouse based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is breast carcinoma.
ERK1 is ubiquitously expressed in various tissues and cells. It is activated by phosphorylation of neighbouring serine and threonine residues by MEK1 and MEK2 in response to diverse stimuli such as growth factors and mitogens.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ERK-1; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase 3; MAP kinase isoform p44; MAPK 1; MAPK3. mitogen-activated protein kinase 3 . HGNC:6877. P44ERK1. P44MAPK. PRKM3. protein kinase; Microtubule-associated protein 2 kinase; mitogen-activated 3 (MAP kinase 3 p44); Mitogen-activated protein kinase 3; p44-ERK1; p44-MAPK
Gene Aliases: ERK-1; ERK1; ERT2; HS44KDAP; HUMKER1A; MAPK3; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM3
UniProt ID: (Human) P27361
Entrez Gene ID: (Human) 5595
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