|Flow Cytometry (Flow)||2-4µg/10^6 cells|
|Western Blot (WB)||0.5-1µg/ml|
|Tested Species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
ERK-1 is a classical MAP kinase involved in intracellular signaling in response to extracellular stimulus. ERK-1 is activated by growth factors, hormones, and phorbol esters. ERKs are widely expressed in different cells, and are involved in control of cell proliferation, cell differentiation, and cytoskeletal regulation. ERK-1 is a serine/threonine kinase which phosphorylates cytoplasmic signaling molecules, cell surface receptors, transcription factors, and microtubule associated proteins upon activation by molecules upstream in the signal transduction pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ERK-1; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; MAPK 1; MAPK 3; Microtubule-associated protein 2 kinase; mitogen-activated 3; mitogen-activated protein kinase 1; Mitogen-activated protein kinase 3; MNK1; p44-ERK1; p44-MAPK; P44MAPK; PRKM3
Gene Aliases: ERK-1; ERK1; ERT2; Esrk1; HS44KDAP; HUMKER1A; MAPK1; MAPK3; MNK1; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM3
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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