|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of human ERK-1 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is breast carcinoma.
ERK-1/extracellular signal-regulated kinase 1/p44 MAP kinase/microtubule-associated protein 2 kinase/myelin basic protein kinase/EGF receptor T669 kinase is ubiquitously expressed in various tissues and cells. It gets activated by phosphorylation of neighboring serine and threonine residues by MEK-1 and MEK-2 in response to diverse stimuli like growth factors and mitogens.
Protein Aliases: ERK-1; ERK1; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAP kinase 3; MAP kinase isoform p44; MAPK 1; Microtubule-associated protein 2 kinase; mitogen-activated protein kinase 1; Mitogen-activated protein kinase 3; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM3
Gene Aliases: ERK-1; ERK1; ERT2; HS44KDAP; HUMKER1A; MAPK3; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM3
UniProt ID: (Human) P27361
Entrez Gene ID: (Human) 5595