|Flow Cytometry (Flow)||0.5ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
|Western Blot (WB)||1:50-1:200|
|Immunohistochemistry (IHC)||See 94 publications below|
|Miscellaneous PubMed (MISC)||See 5 publications below|
|Western Blot (WB)||See 26 publications below|
|Immunoprecipitation (IP)||See 2 publications below|
|Immunocytochemistry (ICC)||See 3 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 3 publications below|
|Tested Species reactivity||Bovine, Human|
|Published species reactivity||Rat , Human , Mouse|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Recombinant human wild-type p53 protein expressed in E. coli|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-12557 targets p53 in FACS, ICC/IF, IHC (P), IP, and WB applications and shows reactivity with Human and bovine samples. This antibody is not suitable for mouse colon tissue in IHC applications.
The MA5-12557 immunogen is recombinant human wild-type p53 protein expressed in E. coli.
This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Antigen NY-CO-13; Cellular tumor antigen p53; mutant tumor protein 53; p53 tumor suppressor; p53 tumor suppressor phosphoprotein; Phosphoprotein p53; TP53; transformation-related protein 53; tumor protein 53; tumor protein p53 (Li-Fraumeni syndrome); Tumor suppressor p53; tumor supressor p53
Gene Aliases: BCC7; BOS_18908; LFS1; P53; TP53; TRP53
Molecular Function: transcription factor
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.