|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:10-1:500|
|Immunohistochemistry (IHC)||See 18 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Rat , Human Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human full-length wild type p53 protein|
|Storage buffer||tissue culture supernatant|
|Contains||15mM sodium azide|
|Storage conditions||4° C|
MA5-14516 targets p53 in IHC (P), ICC/IF, and WB applications and shows reactivity with human samples. This antibody does not cross-react with mouse in immunofluorescent applications.
The MA5-14516 immunogen is recombinant human full-length wild type p53 protein.
This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Antigen NY-CO-13; Cellular tumor antigen p53; mutant tumor protein 53; p53 tumor suppressor; Phosphoprotein p53; TP53; transformation-related protein 53; tumor protein 53; Tumor suppressor p53; tumor supressor p53
Gene Aliases: BCC7; LFS1; P53; TP53; TRP53
UniProt ID: (Human) P04637
Entrez Gene ID: (Human) 7157
Molecular Function: transcription factor
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