|Immunohistochemistry (IHC)||2 µg/ml|
|Western Blot (WB)||0.5-1 µg/ml|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide corresponding to 14 amino acids near the amino terminus of human p53DINP1.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
In Western blot applications, this antibody detects a band at ~27kDa. A suggested positive control is human lung tissue lysate.
PA5-20009 can be used with blocking peptide PEP-0129.
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible gene was identified recently and designated p53DINP1 (for p53-dependent damage-inducible nuclear protein 1) and SIP (for stress induced protein) in human and mouse. A p53DINP1 antisense oligonucleotide inhibits and overexpression of p53DINP1 enhances Ser46 phosphorylation of p53, induction of p53AIP1, and cell death induced by DNA double-strand breaks. p53DINP1 may regulate p53-dependent apoptosis through phosphorylation at Ser46 and induction of p53AIP1. The p53DINP1/SIP gene encodes two proteins of 27 and 18 kDa in human and mouse termed p53DINP1-alpha and p53DINP1-beta or SIP27 and SIP18. p53DINP1/SIP is expressed in many tissues and induced by a variety of stress agents including UV stress, mutagenic stress, heat shock, and oxidative stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: p53-dependent damage-inducible nuclear protein 1; p53-inducible p53DINP1; p53DINP1; SIP, TP53DINP1, TP53INP1A, TP53INP1B, Teap, p53DINP1; stress induced protein; Stress-induced protein; TEAP; Thymus-expressed acidic protein; transformation related protein 53 inducible nuclear protein 1; Tumor protein p53-inducible nuclear protein 1
Gene Aliases: P53DINP1; SIP; Stinp; Teap; TP53DINP1; TP53INP1; TP53INP1A; TP53INP1B; Trp53inp1
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