|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 137-150 of mouse p66a.|
|Storage buffer||whole serum diluted in PBS|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
Suggested positive control: HeLa or RAW.
Methylation of cytosine at CpG dinucleotides is a common feature of many higher eukaryotic genomes. Increasing evidence indicates that recruitement of histone deacetylase complexes by methyl-CpG-binding proteins (MeCP) is a major mechanism of methylated DNA silencing. P66, a zinc finger-containing protein is one of the components of the MeCP1 complex. P66 and p68, the two components of MeCP1 are different forms of the same zinc finger-containing protein and are conserved from C.elegans to humans. Homologs of p66 from different organisms revealed two highly conserved regions, CR1 and CR2. While CR1 is involved in the association of p66 with other MeCP1 components, CR2 plays an important role in targeting p66 and MBD3 to specific loci..
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 1110066C11Rik; BC031407; C80248; GATA zinc finger domain-containing protein 2A; Hp66alpha; MGC29315; MGC36975; p66 alpha; Transcriptional repressor p66 alpha; Transcriptional repressor p66-alpha
Gene Aliases: 1110066C11Rik; BC031407; C80248; GATAD2A; p66alpha; RGD1310596
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