|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||Assay Dependent|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide from the C-terminus of chicken N-cadherin protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is skin, tonsil or squamous epithelium.
Cadherins are calcium dependent cell adhesion molecules, which play important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. In adhesion junctions, cadherins are bound to β and γ catenins, which in turn bind to a catenin, an actin binding protein. Cadherins play important role in tumor invasion and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; calcium-dependent adhesion protein, neuronal; CD325; CDHN; CDw325; N-cadherin; N-cadherin 1; NCAD; Neural cadherin
Gene Aliases: CD325; CDH2; CDHN; CDw325; NCAD
UniProt ID: (Human) P19022
Entrez Gene ID: (Human) 1000
Molecular Function: cadherin
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