|Immunohistochemistry (IHC)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|Western Blot (WB)||See 3 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Immunohistochemistry (IHC)||See 4 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Rat , Fish , Human , Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Cytoskeleton preparation from human A431 carcinoma Cells.|
|Contains||0.08% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This antibody detects cytokeratins 4, 5, 6, 8, 10, 13, 18.
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the
molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.
Reacting with a variety of keratins (4,5,6,8,10,13 and 18) which reacts with a variety of normal reactive and neoplasmic epithelia. Reacting with simple epithelium and both basal and superbasal layers of comfying and non comfying squamous epithelium this antibody is also useful in staining cultured epithelial cell lines. It is useful in differentiating epithelial tumors from non-epithelial tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 58 kDa cytokeratin; Cell proliferation-inducing gene 46 protein; CK-10; CK-13; CK-18; CK-5; CK-6A; CK-6D; CK-8; cytokeratin 10; cytokeratin 13; cytokeratin 18; cytokeratin 4; cytokeratin 6A; cytokeratin 6C; cytokeratin 6D; Cytokeratin-10; Cytokeratin-13; Cytokeratin-18; Cytokeratin-5; Cytokeratin-6A; cytokeratin-6C; Cytokeratin-6D; cytokeratin-6E; Cytokeratin-8; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K10; K13; K18; K5; K6A; K8; kamp-keratin derived antimicrobial peptide; KDAMP; keratin 10, type I; keratin 13, type I; keratin 18, type I; keratin 4, type II; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin 6A, , type II; keratin 6A, type II; keratin 8, type II; keratin K6h; keratin, epidermal type II, K6A; Keratin, type I cytoskeletal 10; Keratin, type I cytoskeletal 13; Keratin, type I cytoskeletal 18; Keratin, type II cytoskeletal 5; Keratin, type II cytoskeletal 6A; Keratin, type II cytoskeletal 8; Keratin-10; Keratin-13; Keratin-18; Keratin-5; Keratin-6A; keratin-6C; Keratin-8; pankeratin; type-II keratin Kb12; type-II keratin Kb4; Type-II keratin Kb5; Type-II keratin Kb6; Type-II keratin Kb8
Gene Aliases: BCIE; BIE; CARD2; CK-18; CK-4; CK-6C; CK-6E; CK-8; CK10; CK13; CK4; CK5; CK6A; CK6C; CK6D; CK8; CYK18; CYK4; CYK8; DDD; DDD1; EBS2; EHK; K10; K13; K18; K2C8; K4; K5; K6A; K6C; K6D; K8; KO; KPP; KRT10; KRT13; KRT18; KRT5; KRT5A; KRT6A; KRT6C; KRT6D; KRT8; PC3; PIG46; WSN1; WSN2
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