Learn the basics of Sanger sequencing

DNA Sequencing by Capillary Electrophoresis, Applied Biosystems Chemistry Guide, 3rd edition (2016, 214 pages)

Description: A perfect handbook for scientists and researchers using Sanger sequencing. In this chemistry guide, you will learn the basic principles of Sanger sequencing from workflow to applications.

DNA Sequencing by Capillary Electrophoresis, Applied Biosystems Chemistry Guide, 3rd edition

Topics include:

  • Sanger sequencing applications
  • DNA template preparation
  • Cycle sequencing
  • Purification of extension products
  • Capillary electrophoresis
  • Data analysis
  • Troubleshooting

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 Oncology
 Genetics/inherited disease
 Infectious disease/Pathogen (Virology/Bacteria)
 HLA
 Ecology/Plant/Animal Species identification/Barcode of Life
 Molecular cloning (plasmid confirmation)
 Gene editing, CRISPR, Transgenic model confirmation
 Genotyping (plants, cell line confirmation)
 Human ID/Forensics/Paternity/Cell line Confirmation
 Epigenetics/Methylation
 Next Generation Sequencing Confirmation
 Non-nucleic acid: Proteomics/Glycan analysis
 Others