Histogram of gated U2-OS cells expressing mCherry protein and labeled with Pacific Blue dye-conjugated rat anti-mCherry antibody (Cat. No. M11238). Samples were acquired and analyzed using 405 nm excitation and 522/31 nm band pass emission filters on an Attune Flow Cytometer.
Due to its improved brightness, superior photostability, and extremely rapid maturation rate, the mCherry monomeric red fluorescent protein is becoming the red fluorescent protein of choice for monitoring physiological processes and detecting transgenic expression. This affinity-purified rat monoclonal antibody can be used to detect native and denatured forms of mCherry or mCherry fusion proteins in western blot analysis, immunoprecipitation, immunocytochemistry, and flow cytometry applications.
Validated for a variety of applications
- Western blotting
- Flow cytometry
In western blot analysis, this mCherry rat monoclonal antibody demonstrates no cross reactivity with Emerald GFP, TagGFP, mKate2, or TagRFP. The same degree of specificity is also demonstrated in immunocytochemistry and flow cytometry applications. Additionally, this antibody has been used successfully in immunoprecpitation.
IHC using an anti-mCherry antibody (fluorescent and stained image). Intestinal tissue from a transgenic mouse expressing mCherry in all tissues. Panel A) Fluorescent image detecting mCherry expression and Panel B) HRP-stained image. Following isolation and fixation in 4% paraformaldehyde, mCherry rat monoclonal antibody (Cat. No. M11217) was used at a 1:15,000 dilution. The ImmPRESS™ Anti-Rat Ig (peroxidase) Polymer Detection Kit (Vector Laboratories) was used according to manufacturer’s instructions; sections were incubated in peroxidase substrate solution until the desired stain intensity developed.
mCherry rat monoclonal antibody conjugates
Fluorescent conjugates of our affinity-purified rat monoclonal antibodies for mCherry fluorescent protein can be used to detect native and denatured forms of mCherry or mCherry fusion proteins in flow cytometry applications. Full-length mCherry was used as the immunogen, and the resulting monoclonal IgG2a-isotype antibody detects denatured and native forms of the protein. Because of its improved brightness, superior photostability, and extremely rapid maturation rate, mCherry monomeric red fluorescent protein is becoming the red fluorescent protein of choice for monitoring physiological processes and detecting transgene expression.
Cell analysis resources
- Cell Imaging Reagent Guide & Protocol App
- Fluorescence and Flow Cytometry Tutorials
- Antibodies for Flow Cytometry
- Antibodies for Western Blotting
- The Molecular Probes Handbook
- Cell Analysis Protocols
- The Cell Culture Basics Handbook
- Cell Analysis Technical Resources
- Labeling Chemistry Selection Tool
- Newsletters & Journals
For Research Use Only. Not for use in diagnostic procedures.