Super Bright 645 versus 650 conjugates

Comparable to Brilliant Violet 650 conjugates

Invitrogen eBioscience Super Bright 645 antibody conjugates for flow cytometry provide:

Find primary antibody conjugates       Find isotype controls

Figure 1. Fluorescence intensity comparison of Super Bright 645 conjugates and Brilliant Violet™ 650 conjugates. (A) Mouse splenocytes stained with anti-CD8a conjugated to Super Bright 645 (red, Cat. No. 64-0081-82) or Brilliant Violet™ 650 conjugate (gray), at the same concentration of antibody. (B) Human peripheral blood cells stained with anti-CD8a conjugated to Super Bright 645 (red, Cat. No. 64-0088-42) or Brilliant Violet™ 650 conjugate (gray), using the same concentration of antibody.

Multiplexing compatibility Buffer Multiplexing considerations
Multiplexing with 1 Super Bright antibody conjugate Standard buffers applicable No special buffer required when only one Super Bright antibody conjugate is used in a panel
Multiplexing with 2 or more Super Bright antibody conjugates Super Bright Staining Buffer Special staining buffer is required prior to addition of Super Bright conjugates to reduce non-specific dye-dye interactions
Multiplexing with 1 or more Brilliant Violet™ antibody conjugates Super Bright Staining Buffer Special staining buffer is required to reduce non-specific dye-dye interactions. Use of the Super Bright Staining Buffer is recommended, but the similar Brilliant Stain Buffer can be substituted

Figure 2. Multiplex flow cytometry experiment. Normal human peripheral blood cells were diluted in Super Bright Staining Buffer, then surface stained with the indicated reagents. Viable cells (determined using a fixable viability stain) were used for analysis to discriminate various T cell subsets.

Viability stain options Product Multiplexing considerations
Fixable LIVE/DEAD fixable dead cell stain kits No compatibility concerns
Non-fixable SYTOX non-fixable dead cell stains

Ready Flow Ready-to-use viability reagents
No compatibility concerns

Compatible with all Ready Flow reagents for viability
Product Multiplexing considerations
UltraComp eBeads microspheres UltraComp eBeads microspheres are compatible but OneComp eBeads are not compatible with violet lasers; the AbC Total Antibody Compensation Bead Kit is also compatible with the Super Bright antibody conjugates.
Staining Target Product Multiplexing considerations
Cytosolic staining
(cytokines)
Intracellular Fixation & Permeabilization Buffer Set No compatibility concerns
Nuclear staining
(transcription factors) 
Foxp3/Transcription Factor Staining Buffer Set No compatibility concerns

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