Immune-Response-Assay

Immuno-oncology (I/O) therapies are considered to be promising anti-cancer treatments and are generating hope and excitement among researchers, clinicians, and patients. The Ion Torrent portfolio of immuno-oncology next-generation sequencing (NGS) assays enables an innovative, multi-dimensional approach to understanding the tumor microenvironment.  Each genomic assay uses the sensitivity of NGS to decipher the hidden biology within precious samples, and can be implemented independently or together for a more holistic view to enhance clinical research study design and positively impact study outcomes.

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Oncomine Immune Response Research Assay

Oncomine Immune Response Research Assay

The Oncomine Immune Response Research panel was carefully selected to monitor the tumor microenvironment (TME). The assay can be used for identification of biomarkers, and to study the mechanism of action and other interactions emanating from combination therapy experiments. In a head-to-head comparison with its competitors, the assay detected lower expressors associated with T-cell receptor (TCR) signaling and check-point inhibitors, thereby allowing researchers to focus on the correct TCR and the most-suited immunotherapy.

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Oncomine TCR Beta-LR Assay

Oncomine TCR Beta-LR Assay

Utilizing a newly developed long-read sequencing technology, the Oncomine TCR Beta-LR Assay is designed to efficiently capture all three complementarity determining regions of the TCR beta chain (CDR1, CDR2, CDR3) with high accuracy. This assay enables key applications, such as predictive or prognostic biomarker discovery, T-cell characterization, and identification of variable gene polymorphisms, using samples such as RNA extracted from whole blood, fresh-frozen tissue, or FACS-sorted cells. The identification of rare and abundant clones can be achieved with as little as 10 ng RNA input. The use of an RNA template allows sequencing of productive and relevant variable (V), diversity (D), and joining (J) rearrangements—improving the identification of rare clones.

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New! Oncomine TCR Beta-SR Assay

New! Oncomine TCR Beta-SR Assay

The newest addition to the Ion Torrent immuno-oncology NGS portfolio, the Oncomine TCR Beta-SR Assay, specifically interrogates the CDR3 region of the TCR beta chain.  Compatible with both FFPE DNA and RNA, this short-read sequencing assay enables characterization of the immune status and detection of T-cell minimal residual disease (MRD) in peripheral blood.   Requiring low sample input, this assay offers a two-day turnaround time complete with superior informatics for accurate clonality and CDR3 TCR beta chain sequence assessment without interference from primer bias.

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Oncomine Tumor Mutation Load Assay

Oncomine Tumor Mutation Load Assay

Tumor Mutation Load or Tumor Mutational Burden (TMB) is rapidly becoming an independent predictor for patient stratification for response to immunotherapy. The Oncomine Tumor Mutation Load Assay covers 1.7 Mb across 409 cancer-driven genes relevant across major cancer types and requires as little as 20 ng of tumor DNA with a three-day workflow and streamlined analysis. The assay highly correlates with exome mutation counts, thereby obviating the need for whole-exome sequencing, allowing for a higher percentage of samples to be evaluated and conserving precious samples for additional biomarker assessment.

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Workflow overview

The Ion Torrent immuno-sequencing solution is designed to help identify markers relevant to immunotherapies using our streamlined sequencing platform and fully integrated informatics solutions to analyze and interpret results. This sample-to-results approach is a superior solution, bringing accuracy, sensitivity, and ease of use to your immuno-oncology research.

RNA Extraction

• Blood

• Fresh-frozen tissue

• Sorted cells

Ion AmpliSeq library

Prepare libraries from either DNA or RNA input depending on your assay requirements

48 Hours

Sequence samples using the Ion GeneStudio S5 System and appropriate Ion 5 Series Chip

Ion reporter software

Perform downstream data analysis with Ion Reporter Software

RNA Extraction

• Blood

• Fresh-frozen tissue

• Sorted cells

Ion AmpliSeq library

Prepare libraries from either DNA or RNA input depending on your assay requirements

48 Hours

Sequence samples using the Ion GeneStudio S5 System and appropriate Ion 5 Series Chip

Ion reporter software

Perform downstream data analysis with Ion Reporter Software

How does Ion AmpliSeq technology work?

How does the Ion S5 System work?