Thermo Scientific offers the most reliable genetic content, superior enzymes and advanced PCR tools to support and facilitate your entire cloning workflow. Our products meet the industry's highest quality control standards and provide performance, accuracy and consistency. Find out more about Thermo Scientific products for each of your cloning workflow steps starting from sample preparation to fragment amplification, DNA modification, electrophoresis and ligation by clicking on the navigation buttons on the menu bar above.

 

Nucleic acid purification: Fast. Efficient.
Convenient.Thermo Scientific GeneJET nucleic acid purification kits are designed for rapid, efficient, and convenient purification of DNA and RNA from a wide range of samples. The kits utilize a proprietary silica-based membrane technology in the form of a convenient spin-column eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. Purified DNA or RNA is ideal for use in all conventional molecular biology procedures.

Features

  • Efficient nucleic acid extraction and high yields
  • High purity of isolated DNA or RNA
  • Simple and fast isolation procedure
  • Convenient silica-based spin column format

Applications

  • Plasmid DNA isolation
  • Genomic DNA isolation
  • Total RNA isolation
  • PCR purification
  • Gel extraction

blood,-mouse, testtube


Learn more

Plasmid DNA Purification

  • GeneJET Plasmid Miniprep Kit
  • GeneJET Plasmid Midiprep Kit
  • GeneJET Plasmid Maxiprep Kit

Purification of DNA Fragments

  • GeneJET Gel Extraction Kit
  • GeneJET PCR Purification Kit

Genomic DNA Purification

  • GeneJET Genomic DNA Purification Kit
  • GeneJET Plant Genomic DNA Purification Mini Kit
  • GeneJET Whole Blood Genomic DNA Purification Mini Kit

Total RNA Purification

  • GeneJET RNA Purification Kit
  • GeneJET Plant RNA Purification Mini Kit
  • GeneJET Whole Blood RNA Purification Mini Kit

Everything for PCR: enzymes, instruments and consumables. In cloning applications, it is crucial to maintain accurate DNA sequences during amplification. Thermo Scientific Phusion High-Fidelity DNA Polymerases have extremely low error rates and set a gold standard for high-fidelity PCR.

  • Extreme fidelity (error rate 4.4 x 10-7 in Phusion HF Buffer).
  • Polymerase speed allows for short extension times (15-30 s/kb).
  • Robust performance, minimal optimization needed.
  • Increased product yields with minimal amounts of enzyme.

Reverse Transcriptases
Maxima Reverse Transcriptases have been developed through in vitro evolution of M-MuLV reverse transcriptase to yield dramatically improved thermostability, robustness and increased synthesis rate compared to wild-type M-MuLV RT.

Thermal Cyclers
Small and fast, the Thermo Scientific Piko Thermal Cycler delivers superior thermal performance in a compact package and enables PCR runs in as little as 15 minutes. For routine PCR, the Thermo Scientific Arktik Thermal Cycler is a reliable and easy-to-use instrument designed to meet everyday requirements in any PCR laboratory.

Consumables
Thermo Scientific PCR plastics are manufactured with industry-leading processes and extensive quality control to ensure maximum PCR performance. The UTW® (ultra-thin wall) PCR plastics allow shorter PCR run times by improving heat transfer.

Learn more

  • Maxima Reverse Transcriptases
  • Phusion High-Fidelity DNA Polymerases
  • Thermo Scientific Solutions for PCR
  • Maxima H Minus Double-Stranded cDNA Synthesis Kit

1 buffer.
176 restriction enzymes.
5 min digestion.

Thermo Scientific FastDigest Enzymes are an advanced line of 176 restriction enzymes designed for rapid DNA digestion. Only FastDigest enzymes are all 100% active in the universal FastDigest and FastDigest Green reaction buffer and are able to digest DNA in 5-15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. As an added convenience, the FastDigest Green buffer allows for direct loading of the reaction mixture on gels.

  • 100% activity of all FastDigest Enzymes in the universal reaction buffers.
  • Complete DNA digestion in 5-15 minutes.
  • Direct loading of reaction products on gels.
  • 176 FastDigest Enzymes available.
  Old Standard Conventional restriction enzymes New Standard FastDigest restriction enzymes
Buffer System Up to 20 Buffers One Universal Buffer
Double/Multiple Restriction Digestion Limited to buffer incompatibility Any digestion combination possible
Reaction Time 1 hour - overnight 5 - 15 minutes
Direct Loading On Gels No Yes
Downstream Applications Partial compatibility 100% buffer compatibility
Star Activity Yes No
Activity Definintion 1 unit of enzyme hydrolyzes 1µg of lambda DNA in 60 minutes in an optimal buffer for an enzyme 1µl of FastDigest enzyme cleaves1 µg of substrate DNA in 5 or 15 minutes of FastDigest buffer

Learn more

  • About FastDigest Restriction Enzymes
  • View the Complete List of FastDigest Restriction Enzymes
  • REsearch™ - find the enzymes you need by name or recognition sequence
  • Recommended buffers for double digest reaction

Modification of DNA ends – fastand efficient. Modification of DNA fragment ends prior to ligation is an essential step to the overall success of a cloning experiment. With the Thermo Scientific Fast DNA End Repair Kit and FastAP Alkaline Phosphatase, modification of DNA ends is fast and effective.

FastAP Alkaline Phosphatase
Our novel FastAP Alkaline Phosphatase is ideal for rapid and efficient dephosphorylation of linearized vector DNA in just 10 min. Complete thermal inactivation of the enzyme in just 5 minutes makes FastAP a faster and more efficient alternative to SAP. This product is also available as part of our Fast Cloning Pack.

Features

  • Fast – 10 min dephosphorylation of all types of DNA ends.
  • Rapid thermal inactivation – 5 min at 75°C.
  • Simultaneous digestion and dephosphorylation of vector DNA - active in restriction enzyme buffers.

Fast DNA End Repair Kit
The new Fast DNA End Repair Kit is designed for fast and efficient DNA fragment blunting and phosphorylation prior to ligation. This kit can be used with all types of double-stranded DNA:

  • fragmented genomic DNA (restriction enzyme digested, nebulized or sonicated)
  • restriction enzyme digested plasmid DNA
  • double stranded cDNA
  • PCR products with 3'-dA overhangs

Features

  • Efficient - blunting and phosphorylation of double-stranded DNA
  • Fast – reaction is complete in 5 minutes
  • Convenient – reaction components are premixed to reduce pipetting steps

Learn more

  • FastAP Alkaline Phosphatase
  • Fast DNA End Repair Kit
  • Thermo Scientific Modifying Enzymes
  • Compatibility Chart


Enlarge

Efficient ligation of DNA fragments following end repair with the Fast DNA End Repair kit

Broad selection of DNA ladders designed for accurate gel migration. Thermo Scientific offers the widest selection of DNA ladders ranging from 10 bp to 48.5 kb for accurate analysis of linear double-stranded DNA in agarose or polyacrylamide gels.

All of our DNA ladders are composed of individual chromatography-purified DNA fragments produced with a patented technology used to prepare pharmaceutical grade plasmid DNA.

Features

  • Broad selection of DNA ladders.
  • Composed of individual chromatography-purified DNA fragments.
  • Bright, sharp bands.
  • Ideal for in-gel DNA quantification.
  • Supplied with extra loading dye for sample DNA.
  • Ready-to-use DNA ladders are stable at room temperature for at least 6 months.
DNA Ladder Line Description Run Time
GeneRuler and O'GeneRuler DNA Ladders 1 kb Ladder, 100 bp ladder and other popular DNA ladders for accurate DNA sizing 45 min
FastRuler DNA Ladders Rapid DNA separation. Ideal for sizing and quantification of PCR products 0-15 min
MassRuler DNA Ladders Accurate in-gel quantification of DNA fragments 45 min
O'RangeRuler DNA Ladders Step-ladders designed for precise sizing of DNA fragments in agarose or non-denaturing polyacrylamide gels 45-90 min
NoLimits Individual DNA Fragments A collection of 36 highly-purified individual DNA fragments ranging in size from 10-20,000 bp individual

1.1% TopVision Agarose, 1X TAE, 7 V/cm, 30 min. NoLimits™ DNA fragments from 300 bp to 10 kb

GeneRuler DNA Ladders

Learn more

  • GeneRuler DNA Ladders
  • FastRuler DNA Ladders
  • MassRuler DNA Ladders
  • O'RangeRuler DNA Ladders
  • NoLimits Individual DNA Fragments

Nucleic acid purification: Fast. Efficient.
Convenient.Thermo Scientific GeneJET nucleic acid purification kits are designed for rapid, efficient, and convenient purification of DNA and RNA from a wide range of samples. The kits utilize a proprietary silica-based membrane technology in the form of a convenient spin-column eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. Purified DNA or RNA is ideal for use in all conventional molecular biology procedures.

Features

  • Efficient nucleic acid extraction and high yields
  • High purity of isolated DNA or RNA
  • Simple and fast isolation procedure
  • Convenient silica-based spin column format

Applications

  • Plasmid DNA isolation
  • Genomic DNA isolation
  • Total RNA isolation
  • PCR purification
  • Gel extraction

blood,-mouse, testtube


Learn more

Plasmid DNA Purification

  • GeneJET Plasmid Miniprep Kit
  • GeneJET Plasmid Midiprep Kit
  • GeneJET Plasmid Maxiprep Kit

Purification of DNA Fragments

  • GeneJET Gel Extraction Kit
  • GeneJET PCR Purification Kit

Genomic DNA Purification

  • GeneJET Genomic DNA Purification Kit
  • GeneJET Plant Genomic DNA Purification Mini Kit
  • GeneJET Whole Blood Genomic DNA Purification Mini Kit

Total RNA Purification

  • GeneJET RNA Purification Kit
  • GeneJET Plant RNA Purification Mini Kit
  • GeneJET Whole Blood RNA Purification Mini Kit

Everything for PCR: enzymes, instruments and consumables. In cloning applications, it is crucial to maintain accurate DNA sequences during amplification. Thermo Scientific Phusion High-Fidelity DNA Polymerases have extremely low error rates and set a gold standard for high-fidelity PCR.

  • Extreme fidelity (error rate 4.4 x 10-7 in Phusion HF Buffer).
  • Polymerase speed allows for short extension times (15-30 s/kb).
  • Robust performance, minimal optimization needed.
  • Increased product yields with minimal amounts of enzyme.

Reverse Transcriptases
Maxima Reverse Transcriptases have been developed through in vitro evolution of M-MuLV reverse transcriptase to yield dramatically improved thermostability, robustness and increased synthesis rate compared to wild-type M-MuLV RT.

Thermal Cyclers
Small and fast, the Thermo Scientific Piko Thermal Cycler delivers superior thermal performance in a compact package and enables PCR runs in as little as 15 minutes. For routine PCR, the Thermo Scientific Arktik Thermal Cycler is a reliable and easy-to-use instrument designed to meet everyday requirements in any PCR laboratory.

Consumables
Thermo Scientific PCR plastics are manufactured with industry-leading processes and extensive quality control to ensure maximum PCR performance. The UTW® (ultra-thin wall) PCR plastics allow shorter PCR run times by improving heat transfer.

Learn more

  • Maxima Reverse Transcriptases
  • Phusion High-Fidelity DNA Polymerases
  • Thermo Scientific Solutions for PCR
  • Maxima H Minus Double-Stranded cDNA Synthesis Kit

1 buffer.
176 restriction enzymes.
5 min digestion.

Thermo Scientific FastDigest Enzymes are an advanced line of 176 restriction enzymes designed for rapid DNA digestion. Only FastDigest enzymes are all 100% active in the universal FastDigest and FastDigest Green reaction buffer and are able to digest DNA in 5-15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. As an added convenience, the FastDigest Green buffer allows for direct loading of the reaction mixture on gels.

  • 100% activity of all FastDigest Enzymes in the universal reaction buffers.
  • Complete DNA digestion in 5-15 minutes.
  • Direct loading of reaction products on gels.
  • 176 FastDigest Enzymes available.
  Old Standard Conventional restriction enzymes New Standard FastDigest restriction enzymes
Buffer System Up to 20 Buffers One Universal Buffer
Double/Multiple Restriction Digestion Limited to buffer incompatibility Any digestion combination possible
Reaction Time 1 hour - overnight 5 - 15 minutes
Direct Loading On Gels No Yes
Downstream Applications Partial compatibility 100% buffer compatibility
Star Activity Yes No
Activity Definintion 1 unit of enzyme hydrolyzes 1µg of lambda DNA in 60 minutes in an optimal buffer for an enzyme 1µl of FastDigest enzyme cleaves1 µg of substrate DNA in 5 or 15 minutes of FastDigest buffer

Learn more

  • About FastDigest Restriction Enzymes
  • View the Complete List of FastDigest Restriction Enzymes
  • REsearch™ - find the enzymes you need by name or recognition sequence
  • Recommended buffers for double digest reaction

Modification of DNA ends – fastand efficient. Modification of DNA fragment ends prior to ligation is an essential step to the overall success of a cloning experiment. With the Thermo Scientific Fast DNA End Repair Kit and FastAP Alkaline Phosphatase, modification of DNA ends is fast and effective.

FastAP Alkaline Phosphatase
Our novel FastAP Alkaline Phosphatase is ideal for rapid and efficient dephosphorylation of linearized vector DNA in just 10 min. Complete thermal inactivation of the enzyme in just 5 minutes makes FastAP a faster and more efficient alternative to SAP. This product is also available as part of our Fast Cloning Pack.

Features

  • Fast – 10 min dephosphorylation of all types of DNA ends.
  • Rapid thermal inactivation – 5 min at 75°C.
  • Simultaneous digestion and dephosphorylation of vector DNA - active in restriction enzyme buffers.

Fast DNA End Repair Kit
The new Fast DNA End Repair Kit is designed for fast and efficient DNA fragment blunting and phosphorylation prior to ligation. This kit can be used with all types of double-stranded DNA:

  • fragmented genomic DNA (restriction enzyme digested, nebulized or sonicated)
  • restriction enzyme digested plasmid DNA
  • double stranded cDNA
  • PCR products with 3'-dA overhangs

Features

  • Efficient - blunting and phosphorylation of double-stranded DNA
  • Fast – reaction is complete in 5 minutes
  • Convenient – reaction components are premixed to reduce pipetting steps

Learn more

  • FastAP Alkaline Phosphatase
  • Fast DNA End Repair Kit
  • Thermo Scientific Modifying Enzymes
  • Compatibility Chart


Enlarge

Efficient ligation of DNA fragments following end repair with the Fast DNA End Repair kit

Broad selection of DNA ladders designed for accurate gel migration. Thermo Scientific offers the widest selection of DNA ladders ranging from 10 bp to 48.5 kb for accurate analysis of linear double-stranded DNA in agarose or polyacrylamide gels.

All of our DNA ladders are composed of individual chromatography-purified DNA fragments produced with a patented technology used to prepare pharmaceutical grade plasmid DNA.

Features

  • Broad selection of DNA ladders.
  • Composed of individual chromatography-purified DNA fragments.
  • Bright, sharp bands.
  • Ideal for in-gel DNA quantification.
  • Supplied with extra loading dye for sample DNA.
  • Ready-to-use DNA ladders are stable at room temperature for at least 6 months.
DNA Ladder Line Description Run Time
GeneRuler and O'GeneRuler DNA Ladders 1 kb Ladder, 100 bp ladder and other popular DNA ladders for accurate DNA sizing 45 min
FastRuler DNA Ladders Rapid DNA separation. Ideal for sizing and quantification of PCR products 0-15 min
MassRuler DNA Ladders Accurate in-gel quantification of DNA fragments 45 min
O'RangeRuler DNA Ladders Step-ladders designed for precise sizing of DNA fragments in agarose or non-denaturing polyacrylamide gels 45-90 min
NoLimits Individual DNA Fragments A collection of 36 highly-purified individual DNA fragments ranging in size from 10-20,000 bp individual

1.1% TopVision Agarose, 1X TAE, 7 V/cm, 30 min. NoLimits™ DNA fragments from 300 bp to 10 kb

GeneRuler DNA Ladders

Learn more

  • GeneRuler DNA Ladders
  • FastRuler DNA Ladders
  • MassRuler DNA Ladders
  • O'RangeRuler DNA Ladders
  • NoLimits Individual DNA Fragments