Ligases catalyze the joining of nucleic acid molecules enzymatically in an ATP-dependent manner. T4 DNA and RNA ligases are members of a large family of ligases and are derived from the T4 bacteriophage representing the most commonly used ligases in cloning protocols.
Thermo Scientific ligase selection table
|T4 DNA Ligase||T4 RNA Ligase|
|Reaction catalyzed||ATP-dependent intra- and intermolecular formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.||ATP-dependent intra- and intermolecular formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxyl termini of poly- and oligonucleotides, ssRNA and ssDNA.|
ss – single-stranded
ds – double-stranded
*Although ATP is routinely used as a phosphate donor, CTP, UTP, GTP, dATP, dTTP are equally effective.
For Research Use Only. Not for use in diagnostic procedures.