Distinguish single-copy differences in DNA copy number

The QuantiGene Plex DNA assay combines branched DNA (bDNA) signal amplification and multianalyte profiling beads (xMAP™) technologies to enable the detection and quantitation of multiple DNA targets simultaneously. The bDNA assay is a hybridization-based method of target-specific DNA quantitation that amplifies signal rather than target DNA.

Unique benefits
  • Accurate—excellent copy number discrimination using branched DNA (bDNA) signal amplification technology 
  • Direct quantitation of target—avoid biases associated with DNA isolation and PCR amplification  
  • Simple—no assay optimization required 
  • High precision—coefficients of variation (CV) are less than 15 percent 
  • Distinguish single-copy differences in DNA copy number 
  • Versatile—great results from any sample: plant and animal tissues, formalin-fixed paraffin-embedded (FFPE) tissue sections, whole blood, cultured cells, isolated DNA, and more

Video: How it works

Single copy gene resolution

QuantiGene Plex DNA Assays can distinguish as little as 20 percent differences in DNA copy number and have the ability to quantitate single copy number variations. The application below shows single-copy gene resolution and the ability to discriminate single-copy differences at the lower end of the linear range of the assay. The QuantiGene Plex DNA Assay has a 3 log linear working range and can detect up to hundreds of DNA copies.

Figure 1: Human BAC fibroblast growth factor receptor 1 (FGFR1) DNA (from 1.3 pg [0.5 copies] to 18.2 pg [7 copies]) was spiked into a constant amount of mouse genomic DNA (180 ng or 30,000 cell equivalents, based on 6 pg mouse DNA/cell). The copy number equivalents of FGFR1 were quantified using the QuantiGene DNA Assay. From 0.5 to 6 FGFR1 DNA copy equivalents per cell were easily distinguished.