The Pathatrix® Auto System has obtained AFNOR certification for the ‘Pathatrix® Auto Salmonella spp.’ Validation data demonstrates equivalence to the reference method ISO 6579 (2002).

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Scope of the AFNOR validation

Food categories:

Meat products: including raw beef, ready-to-eat and ready-to-reheat meat products
Dairy products: including heat-treated milk and UHT, canned or pasteurized dairy products

Salmonella detection workflows:

  • After enrichment, 10 portions of enriched meat or dairy samples are pooled over the Pathatrix® Auto System followed by detection using the MicroSEQ® Salmonella spp real-time PCR detection kit or by detection on selective agar plates according to ISO 6579
  • Positive pooled samples are re-tested individually by reprocessing 10 ml samples of stored individual enriched cultures on the Pathatrix® Auto System, continuing through the chosen detection procedure
  • Independent confirmation testing is performed only on positive individual samples, according to the detection method chosen.

Two protocols have been validated:

  • Pathatrix® Auto Salmonella spp linked to MicroSEQ® Salmonella spp real-time PCR Detection Kit
  • Pathatrix® Auto Salmonella spp linked to Selective Agar Detection

Both protocols allow the screening of the negative samples typically within 2 days, while 3 days are required for the positive samples; compared to 4 days for culture negatives following ISO.

AFNOR validated workflow

The Pathatrix® Auto Instrument is a recirculation immunomagnetic separation system. Using magnetic beads coated with antibodies, the Pathatrix® Auto Instrument quickly concentrates the target organisms such as Salmonella from the enriched samples, and the on-line wash step removes non-target organisms and any PCR inhibitors. This helps reduce the time to results and enables increased pathogen testing at lower costs.

Applying the Pathatrix® Auto System full workflows in your food testing lab enables:

  • more testing at lower costs
  • faster release of your meat and dairy products
  • less false positives due to the removal of matrix effects

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