Choose the Orbitrap MS that's right for your laboratory

  Q Exactive Plus Orbitrap Elite Orbitrap Fusion
Attributes Exactive Plus (EMR) Q Exactive Focus Q Exactive (Plus) Q Exactive
HF
Q Exactive
HF-X
Orbitrap
Elite
Orbitrap Fusion Orbitrap Fusion Lumos
Max resolution (FWHM) @m/z 200 140k 70k 140k (280k Enh R) 240k 340k 500k 1,000k
Mass accuracy, (internal) <1ppm
Scan rate 12Hz 18Hz 40Hz 4Hz 20Hz 40Hz
Polarity switch <1s Not during run < 1.1s
PRM - Yes
Multiplex (precursor/ scan) - - 10
-
10 20
MSn (n=10) - - - - - Yes
ETD option - - - - - Yes
Decision-tree (CID/HCD/ ETD) - - - - - Yes
Synch MS3 - - - - -
-
Yes
Performance and Value

Definition of terms

Resolution: Frequently reported in full width at half maximum (FWHM), resolution is the separation of two mass spectrometry peaks and is calculated as the mass of a peak (M) divided by resolving power (∆M).

Mass accuracy: Expressed in parts per million, this value indicates how much an instrument deviates from a known standard or calibrant.

Scan rate: Measured in hertz, the scan rate of an MS indicates how quickly it scans a mass spectrum.

Polarity switch: The collection of both positive and negative sample ionization data (spectra) by the ion trap during a single MS analysis. Polarity switching is applied to the study of drugs, drug metabolites, pesticides, etc.

PRM: Parallel reaction monitoring (PRM) is a targeted method of high resolution MS quantification in which a full scan of each precursor ion transition is monitored; i.e., all fragments derived from the precursor ion are monitored. In contrast, single reaction monitoring (SRM) performs one transition monitoring at a time.

Multiplex: The ability of a mass analyzer to acquire data from multiple precursor ions in the same scan without compromising high signal-to-noise ratio.

MSn: Also termed tandem MS, the use of multiple stage MS to capture product ion spectra of a defined m/z, where n is the number of recorded product ion stages.

ETD: Electron transfer dissociation (ETD) results in the fragmentation of the peptide backbone of MS samples, simplifying identification of peptide modification sites.

Synchronous MS3: A method of quantitation that involves simultaneous selection of several MS2 precursors for MS3 analysis and is often used during TMT and iTRAQ experiments.


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