Kits de aislamiento de ADN vegetal

El aislamiento de ADN vegetal presenta una serie de retos que requieren kits diseñados específicamente para tratar con los hidratos de carbono, los fenólicos y otros compuestos abundantes en los tejidos vegetales. Las paredes celulares de las plantas pueden ser muy difíciles de interrumpir y los lisados suelen contener cantidades importantes de compuestos como fenólicos, taninos y polisacáridos complejos que pueden afectar a la calidad del ADN y del ARN e inhibir las reacciones posteriores. Life Technologies ofrece una amplia gama de kits, reactivos y dispositivos para el aislamiento, la purificación, el análisis y la cuantificación no solo de ADN genómico de plantas, sino también del ARN total, el ARNm, el microARN o el ARN de transcriptoma.

Ir a Aislamiento de ARN vegetal

Which Plant DNA Isolation Kit is Right for You?

  Bulk buffers for crude extraction of DNA and RNA from plant cells (*) DNA extraction and TaqMan® chemistry-based genotyping combined, <1h to results The most efficient lysis for the largest amount of tissue Most suitable for screening and GMO testing due to automation capabilities Slica-based method for low abundance plant DNA samples Magnetic beads designed to handle low-abundance plant DNA samples
Top Seller yesyes
Starting Material≥ 100 mg2-3mm punch≥ 100 mg50-100 mg100 mg5-10 mg
Yield varies based on starting materialvaries based on starting materialvaries based on starting materialup to 7 µgup to 14.6 µgup to 14.6 µg
Protocol time<15 mins5 mins <60 mins<15 mins<40 mins<40 mins
Plant sample typesCustomer-validated for DNA on a broad range of plant tissues for SNP genotyping as well as corn flour for GMO testingWorks with broad rangeof plant tissues and starting material (leaf punch, needle, seed chips)Broad range of plant tissues (alfalfa and sunflower sprouts, corn husks, soybeans, mushroom, tomato leaves, wheat grass and Arabidopsis thaliana leaves)Some of the many species and tissues are canola, corn, cotton, sunflower, barley, rice (seeds); tomato, cucumber, lettuce, banana (leaves); rose, magnolia (flower); Acer, sycamore, grass, etc.Chloroplasts and broad range of plant tissues (alfalfa and sunflower sprouts, corn husks, soybeans, mushroom, tomato leaves, wheat grass and Arabidopsis thaliana leaves)Chloroplasts and broad range of plant tissues (alfalfa and sunflower sprouts, corn husks, soybeans, mushroom, tomato leaves, wheat grass & Arabidopsis thaliana leaves)
Isolation technologyBulk lysis bufferLysis solutionOrganic extractionChargeSwitch® Chemistry, magnetic beadsSilica spin columnMagnetic beads
Downstream applicationsPCR, real-time PCRReal-time PCRAllAllAllAll
Automatablenoyesnoyesnoyes
High throughput compatibileyesyesyesyesnoyes
High throughput/
automation options
yesyesyesChargeSwitch® gDNA Plant 960 Kit96-well plate50 preps
96 preps
Kit sizes available250 mL100 preps
400 preps
4000 preps
100 mL96 preps
960 preps
50 preps
4 X 96 preps
50 preps
96 preps
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Which Plant RNA Isolation Kit is Right for You?

  Great for difficult samples (conifer tissue & seeds) Rapid & fully automated Quick & easy to use Efficient recovery of microRNA & small RNA
Top Seller   yes
RNA types isolatedlarge RNA molecules onlylarge RNA molecules onlylarge RNA molecules onlySmall & large RNA molecules
Prep time60 minutes<45 minutes<20 minutes30 minutes
Amount of starting materialUp to 1 gUp to 10 mg<250 mg0.5 to 250 mg
Isolation methodHigh purity organic extraction
(requires alcohol precipitation)
Scalable, flexible format with magnetic beadsFast, convenient silica column Highest purity & convenience; includes organic extraction & silica column
(no precipitation required)
High throughput compatible yes  
Compatible products Ambion® Plant RNA Isolation Aid
Removes common plant contaminants
Ambion® Plant RNA Isolation Aid
Removes common plant contaminants
Ambion® Plant RNA Isolation Aid
Removes common plant contaminants
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Other Products of Interest

In addition to DNA and RNA isolation products, Life Technologies offers an expansive portfolio of solutions for analysis and quantitation of plant genomic DNA.

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