These conjugated organelle-specific antibodies join a large selection of organelle specific dyes already available from Thermo Fisher Scientific, and can be used where antibody based approaches may provide an advantage. However, depending upon the experimental design, you can combine with dyes, or traditional primary plus secondary antibody detection.
Organelle antibodies offer broad utility
The conjugated organelle specific antibodies can be used in any application where a pre-conjugated antibody would simplify the experiment. For example instead of GAPDH as a loading control, you could use the signal from actin or tubulin for signal normalization. Alternatively, you could use organelle-specific antibodies in cell fractionation studies, potentially as a control showing that the organelles appeared in the expected cell fraction.
We currently offer over 30 organelle-specific antibodies conjugated to Alexa Fluor 488, Alexa Fluor 555, and Alexa Fluor 647 dyes. Occasionally, we provide more than one potential marker as there may be some sample types or cell treatments where a particular canonical organelle marker is not expressed in the organelle as expected. However, in general these protein targets have been selected due to their known usefulness in examining organelle localization and fractionation.
Find more information on organelle antibodies and search for the organelle antibody you need at thermofisher.com/organelleabs