The centrosome is an organelle that functions as the main microtubule organizing center (MTOC) of the cell and as a regulator of cell cycle progression. Centrosomes are found in higher eukaryotic cells, but not in fungi or plant cells, which use other structures to organize their microtubules. Each centrosome is made up of two centrioles surrounded by a mass of pericentriolar material (PCM), which contains the proteins that allow microtubule nucleation and anchoring. Generally, each centriole is a pinwheel of nine triplet microtubules. Cancer cells often have an abnormal number of centrosomes.

Centrosome marker antibodies detect proteins specific to the centrosome and can aid in the study of the structure and dynamics of the centrosome. Centrosome marker antibodies can also help elucidate the role or roles a protein may play in a number of tasks that are centered in or influenced by the centrosomes. Quality Invitrogen centrosome marker antibodies are available for a variety of research needs.

Centrosome marker antibody targets

  • BUB1B
  • CDK1
  • CDK2
  • CTNNB1
  • GNAI1
  • GNAI2
  • MAP2K1

  • NINL
  • NUMA1
  • PCNT

  • PLK1
  • RAD51
  • SNCG
  • TUBG1

Featured product data

Formalin-fixed, paraffin-embedded human breast carcinoma stained with beta-catenin antibody (Cat. No. PA5-16762) using peroxidase-conjugate and AEC. Note membrane staining of tumor cells.

Immunofluorescent analysis of gamma-tubulin in HeLa (A) and U2-OS cells (B). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Thermo Scientific Blocker BSA (Cat. No. 37525) for 15 minutes at room temperature. Cells were probed with a gamma-tubulin mouse monoclonal antibody (Cat. No. MA1-850) at a dilution of 1:50 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488-conjugated goat anti-mouse secondary antibody (Cat. No. 35503) at a dilution of 1:400 for 30 minutes at room temperature. F-actin (red) was stained with DyLight 547-conjugated phalloidin, and nuclei (blue) were stained with Hoechst 33342 dye (Cat. No. 62249). Images were taken on a Thermo Scientific ArrayScan imager at 20x magnification.

Annotated product references

Cat. No. MA1-850 was used in immunohistochemistry to investigate the neuroprotective effect of synphilin-1 in the A53T alpha-synuclein transgenic mouse model. Human molecular genetics (Jun 2010; 19: 2087) "Synphilin-1 attenuates neuronal degeneration in the A53T alpha-synuclein transgenic mouse model." Smith WW,Liu Z,Liang Y,Masuda N,Swing DA,Jenkins NA,Copeland NG,Troncoso JC,Pletnikov M,Dawson TM,Martin LJ,Moran TH,Lee MK,Borchelt DR,Ross CA

Cat. No. MA1-850 was used in immunocytochemistry and western blot to identify the role of TaSP in microtubule attachment of microbial pathogen. Parasitology research (Apr 2010; 106: 1085) "Schizonts of Theileria annulata interact with the microtubuli network of their host cell via the membrane protein TaSP." Seitzer U,Gerber S,Beyer D,Dobschanski J,Kullmann B,Haller D,Ahmed JS