Beta actin antibodies provide a specific and useful tool for studying the intracellular distribution of beta actin and the static and dynamic aspects of the cytoskeleton. Because it is ubiquitously expressed in all eukaryotic cells, beta actin is frequently used as a loading control.

Beta actin is one of six isoforms of actin that have been identified in mammals. Actin is the primary component of the cytoskeleton, and beta actin is predominantly expressed in non-muscle cells, where it controls cell structure and motility.

Quality Invitrogen beta actin antibodies are available for a variety of research needs. We also offer fluorescent and enzyme-conjugated versions for your added convenience.

Featured product data

Immunohistochemical analysis of beta actin showing staining in the cytoskeleton of paraffin-embedded human breast carcinoma (right), compared with a negative control without primary antibody (left). The staining of the beta actin with the loading control antibody (Cat. No. MA5-15739-BTIN) confirms that proteins in the sample are accessible for binding by other primary antibodies.


 

Western blot analysis of beta actin was performed by loading 30 μg of various cell lysates in wells of a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and probed with an HRP-conjugated beta actin monoclonal antibody (Cat. No. MA5-15739-HRP). Chemiluminescent detection was performed using SuperSignal West Pico Substrate (Cat. No. 34080).


Flow cytometry analysis of beta actin in A431 cells (green) compared to an isotype control (blue). Cells were harvested and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 minutes at room temperature and incubated with a beta actin loading control antibody (Cat. No. MA5-15739-BTIN). Cells were then incubated for 40 min at room temperature in the dark with a secondary antibody conjugated to DyLight 488 dye, and resuspended in PBS for FACS analysis.
Cell: A431
Concentration: 2ug/test (100ul)
Theory location: Cytoplasm


 


Western blot analysis of beta actin was performed by loading various cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a low-fluorescence PVDF membrane and probed with a beta actin monoclonal antibody conjugated to Alexa Fluor 488 dye (Cat. No. MA1-140-A488).


Annotated product references

MA5-15739 was used in western blotting to develop a 3D model for studying tumors by co-culturing HT-29 tumor spheroids with CCD-18co fibroblasts. Kim SA, Lee EK, Kuh HJ (2015) Co-culture of 3D tumor spheroids with fibroblasts as a model for epithelial-mesenchymal transition in vitro. Exp Cell Res 335: 187–196.

MA5-15739 was used in western blotting to investigate the role of FoxO1 during adipocyte differentiation and adipogenesis. Zou P, Liu L, Zheng L et al. (2014) Targeting FoxO1 with AS1842856 suppresses adipogenesis. Cell Cycle 13: 3759–3767.

MA5-15739 was used in western blotting to study the control of excessive reactive astrogliosis and improved functional recovery in a rat model of spinal cord injury treated with an EGFR tyrosine kinase inhibitor. Li ZW, Li JJ, Wang L et al. (2014) Epidermal growth factor receptor inhibitor ameliorates excessive astrogliosis and improves the regeneration microenvironment and functional recovery in adult rats following spinal cord injury. J Neuroinflammation 11: 71.

MA5-15739 was used in western blotting to investigate the mechanisms underlying dysregulated osteoclastogenesis in HIV. Lafferty MK, Fantry L, Bryant J et al. (2014) Elevated suppressor of cytokine signaling-1 (SOCS-1): a mechanism for dysregulated osteoclastogenesis in HIV transgenic rats. Pathog Dis 71: 81–89.

MA5-15739-HRP was used in western blotting to study the formation of amylin amyloid deposits in the cerebrovascular system and the implications for Alzheimer's disease. Jackson K, Barisone GA, Diaz E et al. (2013) Amylin deposition in the brain: A second amyloid in Alzheimer disease? Ann Neurol 74: 517–526.


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