Beta tubulin is a 55 kDa protein that forms a heterodimer with alpha tubulin to make tubulin, the primary component of microtubules. Microtubules mediate many cell movements, including intracellular transport, cilliary and flagellar beating, chromosome alignment in mitosis and meiosis, and structural support of the cytoskeleton. As beta tubulin is ubiquitously expressed in eukaryotic cells, it is often used as a loading control. 

Quality Invitrogen beta tubulin antibodies are available for a variety of research needs. We also offer fluorescent and enzyme-conjugated versions for your added convenience. 

Featured product data

Immunofluorescence analysis of beta tubulin (red) in HeLa cells. Cells were stained with a beta tubulin monoclonal antibody conjugated to Alexa Fluor 647 dye (Cat. No. MA5-16308-A647). Nuclei (were stained with Hoechst™ 33342 dye (blue). Image was obtained at 20x magnification.


 

 

Western blot analysis of beta tubulin was performed by loading various cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a low fluorescence PVDF membrane and probed with a beta tubulin monoclonal antibody conjugated to Alexa Fluor 555 dye (Cat. No. MA5-16308-A555).


Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tubules stained using a beta tubulin polyclonal antibody (Cat. No. PA1-41331). 



Flow cytometry analysis of beta tubulin in NIH3T3 cells (green) compared to an isotype control (blue).
Cells were incubated with a beta tubulin loading control antibody (Cat. No. MA5-16308-D650). Cells were then incubated for 40 min at room temperature in the dark using a secondary antibody conjugated to DyLight 488 dye, and re-suspended in PBS for FACS analysis.
Cell: 3T3
Concentration: 1 μg/test (100 μL)
Theory location: cytoplasm


Annotated product references

PA1-16947 was used in western blotting to determine if methylhonokiol analogs inhibit the expression of inflammatory genes in macrophages and adipocytes. Kim S, Ka SO, Lee Y et al. (2015) The new 4-O-methylhonokiol analog GS12021 inhibits inflammation and macrophage chemotaxis: role of AMP-activated protein kinase a activation. PLoS One 10(2): e0117120.

MA5-16308 was used in western blotting to study the differential effects of a high-fat diet and caloric restriction on murine hypothalamic CNTF signaling. Severi I, Perugini J, Mondini E et al. (2013) Opposite effects of a high-fat diet and calorie restriction on ciliary neurotrophic factor signaling in the mouse hypothalamus. Front Neurosci 7: 263.

PA1-16947 was used in western blotting to study the mechanisms underlying the inhibitory effects of butein on adipocyte inflammation and macrophage chemotaxis. Wang Z, Lee Y, Eun JS, Bae EJ (2014) Inhibition of adipocyte inflammation and macrophage chemotaxis by butein. Eur J Pharmacol 738: 40–48.

PA1-41331 was used in western blotting to determine if Reg3a improves islet engraftment. Ding Y, Xu Y, Shuai X et al. (2014) Reg3a overexpression protects pancreatic beta-cells from cytokine-induced damage and improves islet transplant outcome. Mol Med 20: 548–558.

MA5-16308 was used in western blotting to study the mechanism by which PTPN14 modulates YAP activity and the significance for oncogenesis. Michaloglou C, Lehmann W, Martin T et al. (2013) The tyrosine phosphatase PTPN14 is a negative regulator of YAP activity. PLoS One 8(4): e61916.


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