Immunofluorescent analysis of c-Myc (green) in HEL 11.4 induced IPS cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton™ X-100 for 15 minutes at room temperature. Cells were probed with a c-Myc monoclonal antibody (Cat. No. MA1-980) at a dilution of 1:200 overnight at 4°C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.
Cat. No. MA1-980-1MG was used in western blot to study the activation of the PRC-dependent stress program by apoptosis and senescence and its role in the response to cellular dysfunction. Gleyzer N, Scarpulla RC (2013) Activation of a PGC-1-related coactivator (PRC)-dependent inflammatory stress program linked to apoptosis and premature senescence. J Biol Chem 288:8004– 8015.
Cat. No. MA1-21316 was used in immunohistochemistry and western blot to study the cellular pathology of a CHMP2B missense mutation associated with neurodegeneration. Han JH, Ryu HH, Jun MH et al. (2013) The functional analysis of the CHMP2B missense mutation associated with neurodegenerative diseases in the endo-lysosomal pathway. Biochem Biophys Res Commun 421:544–549.
Cat. No. MA1-21316 was used in immunoprecipitation to study the interaction of the viral trans-frame P3N-PIPO protein with host PCaP1 and its role in potyvirus cell-to-cell movement. Vijayapalani P, Maeshima M, Nagasaki-Takekuchi N et al. (2012) Interaction of the trans-frame potyvirus protein P3N-PIPO with host protein PCaP1 facilitates potyvirus movement. PLoS Pathog 8:e1002639.