Immunohistochemistry Information

Find protocols, educational webinars, scientific posters and more to help you design your immunohistochemistry experiments.

Immunohistochemistry features

BioProbes article

Bioprobes

Immune checkpoint antibodies for flow cytometry, IHC, and functional bioassays

This article features an overview of the current antibodies available to act as immune checkpoints for T cell defense against invading tumors.

Scientific poster

scientific-poster-immi

Incorporating histological analysis into existing high-content imaging

To address the need for quantitative colorimetric imaging, we have developed a method for analyzing histological samples on a high-content imaging instrument using a specialized brightfield unit


Immunohistochemistry learning resources

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Type Title Categories
BioProbes article (2008) Nuclear receptor antibodies: A core technology  antibodies, cell signaling, ELISA, gene expression, immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), ion flux/ion channels, western blotting
BioProbes article (2009) Novel recombinant monoclonal antibodies—ABfinity technology ensures performance  antibodies, cell signaling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC), protein quantitation, sample preparation, western blotting
BioProbes article (2009) Baculoviral transduction of human neurons  chemiluminescence, ELISA,fluorescent dyes, gene expression, immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), neuroscience, stem cell research, western blotting
BioProbes article (2009) Fluorescence-based alternatives to chromogenic immunohistochemistry  antifades, cancer, fluorescent dyes, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC), particles, protein quantitation, sample stability
BioProbes article (2009) Specific detection of gap junction proteins  Alexa Fluor, antibodies, fluorescent dyes, fluorescence microscopy/fluorescence imaging, immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), protein detection
BioProbes article (2009) Is it time to go direct? IgG antibody labeling methods as versatile as your imagination  Alexa Fluor, antibody labeling, flow cytometer/flow cytometry, fluorescent dyes, immunocytochemistry (ICC), immunohistochemistry (IHC), nanocrystals, Qdot, sample preparation, Zenon
BioProbes article (2015) Applications for therapeutic monoclonal antibodies—Tools for studying antibody internalization and trafficking antibodies, antibody-drug conjugates, antibody labeling, drug research, endocytosis/phagocytosis, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunohistochemistry (IHC), internalization, pH detection
BioProbes article (2016) Histological analysis using the CellInsight CX7 HCA Platform—Quantitative imaging of histological samples CellInsight, high content analysis, immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC)
BioProbes article (2016) Antibodies for pluriptent stem cells and neural lineage cells—Light up neural diffentiation pathways antibodies, high content analysis, immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunophenotyping, neuroscience, stem cell research
BioProbes article (2016) Click-iT EdU and Click-iT TUNEL Colorimetric Assays for immunohistochemistry—Proliferating and apoptotic cells revealed apoptosis, brightfield microscopy, cell proliferation, Click-iT, colorimetric,fixed-cell imaging, immunohistochemistry (IHC)
BioProbes article (2017) Effectively monitor antibody internalization and trafficking—Improved labeling tools for antibody–drug conjugate screening and characterization antibodies, antibody-drug conjugates, antibody labeling, drug research, endocytosis/phagocytosis, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunohistochemistry (IHC), internalization, pH detection
BioProbes article (2017) Harness immune checkpoints to combat tumors—Immune checkpoint antibodies for flow cytometry, IHC, and functional bioassays antibodies, flow cytometer/flow cytometry, immunofluorescence (IF), immunohistochemistry (IHC), immunology, immuno-oncology
Pierce Protein Methods Overview of immunohistochemistry (IHC) antibodies, fixation, immunohistochemistry (IHC)
Pierce Protein Methods Immunohistochemistry methods articles antibodies, fixation, immunohistochemistry (IHC)
Protocol Immunohistochemical staining of formalin-fixed paraffin-embedded tissues general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC frozen tissue—Indirect method (purified) general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC frozen tissue—Indirect method (biotin) general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC frozen tissue—Direct method general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC FFPE Tissue Trypsin Digestion—Direct Method general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC FFPE tissue trypsin digestion antigen retrieval—Indirect method general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC FFPE tissue low pH antigen retrieval—Indirect method general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC FFPE tissue low pH antigen retrieval—Direct method general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC FFPE tissue high pH antigen retrieval—Indirect method general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC FFPE tissue high pH antigen retrieval—Direct method general ab staining, imaging, immunohistochemistry (IHC)
Scientific poster (2015) Colorimetric dual labeled EdU/BrdU technology demonstrates contextual information and dynamics of proliferation in tissue  apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC)
Scientific poster (2016) Correlating internalization and potency to accelerate antibody discovery and development brightfield microscopy, colorimetric, endocytosis and phagocytosis, EVOS,fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunohistochemistry (IHC)
Scientific poster (2016) Incorporating histological analysis into existing high content imaging  antibodies, ArrayScan, brightfield microscopy, fluorescence microscopy/fluorescence imaging, high content analysis, immunohistochemistry (IHC)
Scientific poster (2016) Click colorimetric EdU proliferation and TUNEL: Click chemistry for brightfield microscopy apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC)
Video Immunohistochemistry—Tips and tricks for weak or no stain Immunohistochemistry application, offers a spatial visualization and location of an antigen in tissue sections  antibodies, brightfield microscopy, colorimetric, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC), secondary detection
Video Immunohistochemistry—Tips and tricks for high background Immunohistochemistry application offers a spatial visualization and location of an antigen in tissue sections. This video offers troubleshooting tips for issues falling into the "high background" category for IHC  antibodies, brightfield microscopy, colorimetric, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC), secondary detection