Th17 Differentiation Functional Assay
Th17 cells are a subset of activated CD4+ T cells that are responsive to IL-1R1 and IL-23R signaling. They act as a bridge between adaptive and innate immunity where they promote neutrophil activation, immunity to pathogens, and inflammation. Through the study of IL-23, it was discovered that an alternate Th cell subset promotes chronic inflammation and tissue damage. Th17 cells were classified as an additional effector CD4+ T cell subset based on their independence from the transcription factors GATA3 and T-bet and the cytokines IFNγ and IL-4, used to define Th1 and Th2, respectively. While Th17 cell differentiation is driven by TGFβ and IL-6, in vitro it has been shown that IL-1β and IL-23 are also necessary in vivo for Th17 development. Th17 differentiation is driven and regulated by the lineage-defining transcription factors AHR, BATF, IκBζ, IRF4, c-Maf, RORα, RORγt, and STAT3. STAT3 is critical for Th17 differentiation and directly regulates the locus encoding IL-17 and is necessary for the expression of many transcription factors involved in Th17 differentiation. Beyond that, IL-23 is required for Th17 expansion and stabilization. Cytokines such as IFNγ, IL-27 and IL-4 are known to inhibit Th17 differentiation.
Relevant recombinant proteins
- IL-1 beta
- Involved in early Th17 differentiation
- Upregulates RORγt and IRF4
- Helps maintain Th17 cytokine profile post-polarization
- Decreases the ability of de-differentiation and plasticity in Th17 cells
- Induces expression of the characteristic Th17 cytokines
- Essential for the survival and expansion
For Research Use Only. Not for use in diagnostic procedures.