Apoptosis Information

Many different approaches are used in the study of programmed cell death, or apoptosis. Cell death cascades are complex and dynamic, underscoring the importance of a multi-parametric approach for accurate assessment. This page features application notes, webinars and other useful educational resources to help you navigate the complex world of apoptosis research.

Apoptosis features

BioProbes article

BioProbes article

Multiplexable Click-iT Plus TUNEL Assays for in situ apoptosis detection

Here we describe the limitations of conventional TUNEL assays and introduce the Click-iT Plus TUNEL Assays for In Situ Apoptosis Detection. Click-iT Plus technology enables you to perform specific and sensitive TUNEL assays that can be multiplexed with other fluorescence-based cell function assays, including those that incorporate fluorescent proteins and a variety of dyes.

On-demand webinar

The meaning of life at the cellular level: detecting apoptosis with fluorescence

In this webinar, Thermo Fisher Scientific scientists will demonstrate several solutions using fluorescent probes to study key components of the apoptotic machinery.


Apoptosis learning resources

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Type Title Categories
Application note (2011) Apoptosis detection: Apoptosis assays for the Attune Acoustic Focusing Cytometer  Alexa Fluor, apoptosis, Attune/Attune NxT, cell structure-mitochondria, cell structure-plasma membrane, flow cytometer/flow cytometry, fluorescent dyes
Application note (2013) Multiplexed mitosis and apoptosis analysis  antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, particles
Application note (2014) Elucidating the mechanism of cell death through the use of high content analysis  apoptosis, autophagy, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
Application note (2015) Fluorescent apoptosis evaluation on the Countess II FL Automated Cell Counter apoptosis, automated cell counter, cell counting, Countess, EVOS, fluorescent dyes, viability
Application note (2017) Flow cytometry analysis of dose response for apoptosis induction apoptosis, Attune/Attune NxT, cancer, flow cytometer/flow cytometry
BioProbes article (2006) Analyzing cell culture health and concentration  apoptosis, cell health, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, microplate reader, particles, viability
BioProbes article (2007) Looking for apoptosis?  antibodies, apoptosis, colorimetric, ELISA, flow cytometer/flow cytometry,fluorescent dyes, immunocytochemistry (ICC), protein detection, western blotting
BioProbes article (2007) Investigating apoptosis-induced loss of SYTO dye fluorescence  apoptosis, cell structure-mitochondria, enzyme activity detection, flow cytometer/flow cytometry, fluorescent dyes
BioProbes article (2007) Multiparametric approaches for studying apoptosis  Alexa Fluor, antibodies, apoptosis, cell structure-mitochondria, cell structure-plasma membrane, enzyme activity detection, flow cytometer/flow cytometry, fluorescent dyes, viability
BioProbes article (2008) Recent news in neurodegeneration  ELISA, apoptosis, cell health, fluorescent dyes, neuroscience, proteins, viability, western blotting
BioProbes article (2009) A growing list of tools for the violet laser  Alexa Fluor, apoptosis, cell cycle, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, nanocrystals, Qdot, viability
BioProbes article (2009) Dead cell stains in flow cytometry: A comprehensive analysis  apoptosis, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, viability
BioProbes article (2009) A pHrodo dye-based method for monitoring the phagocytic internalization of apoptotic cells  apoptosis, fluorescent dyes, fluorescence microscopy/fluorescence imaging, pH detection, phagocytosis
BioProbes article (2009) TUNEL vision  apoptosis, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, sample preparation, sample stability
BioProbes article (2010) Cells under stress—Fluorescence-based tools for investigating autophagy apoptosis, autophagy, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2010) A ratiometric approach to apoptosis detection by flow cytometry apoptosis flow cytometer/flow cytometry, fluorescence
BioProbes article (2011) Introducing the Tali Image-Based Cytometer—Three-parameter population analysis right from your bench apoptosis, fluorescent proteins, viability 
BioProbes article (2011) Bringing cell death to light—CellEvent Caspase-3/7 Substrate and the multiplex analysis of apoptosis apoptosis, ArrayScan, CellEvent, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
BioProbes article (2011) No-wash imaging of apoptosis—CellEvent Caspase-3/7 Green Detection Reagent for apoptosis apoptosis, ArrayScan, caspase substrates, CellEvent, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2013) ReadyProbes ready-to-use imaging reagents for brilliant results apoptosis, cell structure-all, fluorescence microscopy/fluorescence imaging, high content analysis, reagents, Imaging sample preparation, viability
BioProbes article (2013) Checking vital signs: Don't let dead cells mislead you—Molecular Probes cell health assays for flow cytometry apoptosis, caspase substrate, cell cycle, cell health, cell proliferation, cell tracking, Click-iT, flow cytometer/flow cytometry,oxidative stress, viability
BioProbes article (2015) Multiplexable Click-iT Plus TUNEL assays for in situ apoptosis detection—Visualize apoptosis in context apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2015) Journal Club—Multiparameter detection of early apoptosis markers without compensation apoptosis, Attune/Attune NxT, cell structure-mitochondria, flow cytometer/flow cytometryimmunophenotyping
BioProbes article (2016) Click-iT EdU and Click-iT TUNEL Colorimetric Assays for immunohistochemistry—Proliferating and apoptotic cells revealed apoptosis, brightfield microscopy, cell proliferation, Click-iT, colorimetric,fixed-cell imaging, immunohistochemistry (IHC)
BioProbes article (2017) Journal club—Measurement and characterization of apoptosis by flow cytometry flow cytometer/flow cytometry, apoptosis, fluorescent dyes
Molecular Probes Handbook Assays for apoptosis and autophagy—Section 15.5 apoptosis, autophagy, fluorescent dyes, fluorescence microscopy/fluorescence imaging, flow cytometer/flow cytometry
Protocol Pharmacological induction of apoptosis with camptothecin cell vitality, flow cytometry, high content analysis, imaging, microplate
Scientific poster (2006) Utility of 405 nm-excitable dyes in high content screening using an Acumen Explorer Microplate Cytometer  Acumen Explorer, apoptosis, cell cycle, cell health, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, violet laser-excited reagents
Scientific poster (2008) Characterization of DNA content, cyclin B1 and phosphorylated histone H3 with direct S-phase using EDU incorporation in multiparameter testing of cell lines with cell cycle blocking agents  Alexa Fluor, antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
Scientific poster (2008) Novel Click-IT TUNEL Assay for detection of cell death  Alexa Fluor, antibodies, apoptosis, Click-iT, cytotoxicity, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, high content analysis, immunocytochemistry (ICC), viability
Scientific poster (2009) A non-radioactive method for detecting nascent protein synthesis by high-content imaging utilizing click chemistry  Alexa Fluor, apoptosis, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, protein detection
Scientific poster (2009) A simple, fast and quantitative single-step dead-cell indicator for flow cytometry  apoptosis, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, nucleic acid quantitation, viability
Scientific poster (2009) Dead cell stains in flow cytometry: A comprehensive analysis  apoptosis, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, viability
Scientific poster (2009) Click chemistry-based assays for fluorescence microscopy and high content imaging  Alexa Fluor, apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid detection, protein detection, viability
Scientific poster (2010) Simultaneous analysis of cell death mechanisms and oxidative stress using Molecular Probes next generation reagents for imaging and flow cytometry  apoptosis, autophagy, caspase substrates, flow cytometry/flow cytometer, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, oxidative stress
Scientific poster (2010) Acoustic focusing cytometry: Sensitivity and throughput  apoptosis, Attune/Attune NxT, cell cycle, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, phagocytosis
Scientific poster (2010) Simultaneous analysis of cell death mechanisms and oxidative stress using live cell fluorescence microscopy  apoptosis, ArrayScan, BacMam technology, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, microplate reader, oxidative stress
Scientific poster (2011) Simultaneous analysis of cell death mechanisms and oxidative stress using high content imaging  Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress
Scientific poster (2011) Validation of high content imaging and analysis assays for cell health and cytotoxicity  antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, cell health, cell proliferation, CellLight, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, pH detection
Scientific poster (2011) The next generation of cell-based imaging assays for apoptosis, autophagy and oxidative stress from Molecular Probes  Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress
Scientific poster (2013) Monitor caspase 3/7 activity without cell fixation: A novel apoptosis reagent from Molecular Probes  Alexa Fluor, apoptosis, Attune/Attune NxT, caspase substrates, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry
Scientific poster (2013) Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health  apoptosis, ArrayScan, caspase substrates, cell health, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, microplate reader, oxidative stress
Scientific poster (2015) Colorimetric dual labeled EdU/BrdU technology demonstrates contextual information and dynamics of proliferation in tissue  apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC)
Scientific poster (2015) Visualizing the life and death of cells: novel probes and platforms  apoptosis, ArrayScan, autophagy, cell death, CellInsight, fluorescence microscopy/fluorescence imaging, high content analysis
Scientific poster (2016) Click colorimetric EdU proliferation and TUNEL: Click chemistry for brightfield microscopy  apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC)
Video CellEvent Caspase 3/7 reagent and apoptosis detection  
HeLa cells were loaded with 50 nM TMRM (red) followed by 5 μM CellEvent Caspase 3/7 Substrate (green). Cells were then treated with 0.5 μM staurosporine to induce apoptosis. TMRM detects the mitochondrial membrane potential and the signal disappears as the apoptosis onsets. CellEvent detects apoptosis, with green signal.
apoptosis, cell health, CellEvent, fluorescence microscopy/fluorescence imaging, live-cell imaging
Video Click-iT Plus TUNEL apoptosis assays  
The Click-iT Plus TUNEL assay detects apoptotic cells in tissue and cultured cell samples through the use of a small, highly specific labeling moiety and a bright fluorescent dye. After incorporation of the labeling moiety into DNA fragments, detection is achieved through a catalyzed "click" reaction using conditions mild enough to preserve the fluorescent signal from GFP or RFP.
apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging
Video Time-lapse of cell migration and apoptosis during angiogenesis  
Angiogenesis pseudo-tube formation and apoptosis shown with time-lapse imaging. The time-lapse images were captured and assembled using an EVOS FL Auto Imaging System equipped with the EVOS Onstage Incubator.
angiogenesis, apoptosis, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, live-cell imaging, onstage incubator
Webinar The meaning of life at the cellular level: detecting apoptosis with fluorescence  
In this webinar, Thermo Fisher Scientific scientists will demonstrate several solutions using fluorescent probes to study key components of the apoptotic machinery. The presentation includes: An overview of the features of apoptosis Key parameters that can be measured to assay apoptosis A comprehensive guide to available labeling and detection technologies for apoptosis research Tips and tricks to best implement those technologies
apoptosis, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent proteins
Webinar An introduction to flow cytometric analysis, Part 2: Cell viability and apoptosis analysis  
In this free webinar, we will discuss flow cytometric analysis of apoptosis and identification of dead cells. Changes introduced by apoptosis can be tested with numerous assays measuring Membrane structure, Mitochondrial function, Metabolism, Caspase activity, Membrane integrity, and DNA fragmentation. We will also discuss dead cell identification using traditional impermeant nucleic acid dyes.
apoptosis, cell cycle, cell proliferation, flow cytometer/flow cytometry, multicolor flow cytometry, viability
Webinar An introduction to flow cytometric analysis, Part 1: Cell proliferation analysis  
In this 2-part series, we will give an overview of tools and techniques using Invitrogen reagents for flow cytometric analysis of cell proliferation, viability, vitality, and apoptosis.
apoptosis, cell cycle, cell proliferation, flow cytometer/flow cytometry, multicolor flow cytometry, viability