Endocytosis and Phagocytosis Information

Endocytosis and phagocytosis play an important role in cell metabolism and function. Here you will find resources to help you understand how you can track and monitor these processes.

Endocytosis and phagocytosis features

On-demand webinar

The meaning of life at the cellular level: Visualizing membrane trafficking

This webinar provides an overview of the processes involved in membrane trafficking, along with strategies to investigate them using fluorescence microscopy. 

Scientific poster

Correlating internalization and potency to accelerate antibody discovery and development

In this scientific poster presented at a Keystone Symposium, we present experimental data demonstrating the use of pH-sensitive fluorophores to monitor the three primary mechanisms of endocytosis in live cells.


Endocytosis and phagocytosis learning resources

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Type Title Categories
Application note (2015) Guide to setting up hypoxic conditions on the EVOS FL Auto Imaging System with Onstage Incubator EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, live-cell imaging, onstage incubator, phagocytosis
Application note (2015) Quantitation of proliferating cells with the EVOS FL Auto Imaging System antibodies, antibody labeling, ArrayScan, cell proliferation, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, onstage incubator, phagocytosis
Application note (2015) No-wash, no-lyse detection of phagocytic cells via a pHrodo BioParticles functional assay in human whole blood on the Attune NxT Flow Cytometer antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immune system, immunophenotyping, particles, pH detection
BioProbes article (2007) Putting the pH in phagocytosis  flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, microplate reader, multicolor flow cytometry, live-cell imaging, pH detection, phagocytosis, reagents, sample preparation
BioProbes article (2009) Traffic lights: Illuminating internalization pathways  drug discovery, flow cytometer/flow cytometry, fluorescent dyes, particles, pH detection, phagocytosis, reagents
BioProbes article (2009) A pHrodo dye-based method for monitoring the phagocytic internalization of apoptotic cells  apoptosis, fluorescent dyes, fluorescence microscopy/fluorescence imaging, pH detection, phagocytosis
BioProbes article (2011) Endocytosis in disease and cell health cell health, cell structure, CellLight, endocytosis and phagocytosis, fluorescence microscopy/fluorescence imaging, high content analysis, pH detection
BioProbes article (2012) Tracking internalization in live cells with pHrodo dyes endocytosis/phagocytosis, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, internalization, live-cell imaging, pHrodo
BioProbes article (2015) Applications for therapeutic monoclonal antibodies—Tools for studying antibody internalization and trafficking antibodies, antibody-drug conjugates, antibody labeling, drug research, endocytosis/phagocytosis, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunohistochemistry (IHC), internalization, pH detection
BioProbes article (2017) Effectively monitor antibody internalization and trafficking—Improved labeling tools for antibody–drug conjugate screening and characterization antibodies, antibody-drug conjugates, antibody labeling, drug research, endocytosis/phagocytosis, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunohistochemistry (IHC), internalization, pH detection
Molecular Probes Handbook Probes for following receptor binding and phagocytosis—Section 16.1 cell structure-plasma membrane, fluorescence microscopy/fluorescence imaging, fluorescent dyes, internalization, membrane trafficking, phagocytosis, receptor binding, vesicle
Molecular Probes Handbook Fluorescent and biotinylated dextrans—Section 14.5 cell lineage tracing, cell loading, dextran conjugates, endocytosis, fluid transport, fluorescent dextrans, intercellular communications, membrane permeability, neuronal tracing
Molecular Probes Handbook Choosing a tracer—Section 14.1 cell lineage tracing, cell tracing, dextran conjugates, endocytosis, fluid transport, intercellular communications, membrane permeability, neuronal tracing, polar tracers, protein conjugates
Scientific poster (2009) High content imaging of endocytosis & phagocytosis using pHrodo conjugates  Alexa Fluor, ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, pH detection, phagocytosis, viability
Scientific poster (2010) Acoustic focusing cytometry: Sensitivity and throughput  apoptosis, Attune/Attune NxT, cell cycle, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, phagocytosis
Scientific poster (2010) New fluorescent probes for live-cell imaging  autophagy, cell cycle, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging, pH detection, phagocytosis
Scientific poster (2013) Cells coming to life: Tools for visualizing immune response  Attune/Attune NxT, endocytosis/phagocytosis, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, immunophenotyping, live-cell imaging, pH detection
Scientific poster (2013) Illuminating endocytosis with targeted pH-sensitive fluorescent compounds  Alexa Fluor, ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, pH detection, phagocytosis, viability
Scientific poster (2014) New fluorescent probes and sensors for visualizing endocytosis, lysosomal dynamics and autophagy  autophagy, cell structure-lysosomes, endocytosis/phagocytosis, autophagy, fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader
Scientific poster (2015) A no lyse, no-wash approach to characterizing phagocyte phenotype and function in whole human blood on the Attune NxT Flow Cytometer  Attune/Attune NxT, cell cycle, flow cytometer/flow cytometry, immunophenotyping, no lyse, phagocytosis, whole blood
Scientific poster (2016) Correlating internalization and potency to accelerate antibody discovery and development brightfield microscopy, colorimetric, endocytosis and phagocytosis, EVOS,fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunohistochemistry (IHC)
Webinar The meaning of life at the cellular level: Visualizing membrane trafficking 
Membrane trafficking underlies the acquisition and release of cellular material as well as the transport of macromolecules from the site of cellular synthesis to their functional location. Thermo Fisher Scientific offers a number of solutions for researchers using fluorescent probes to study key components of the endocytotic and secretory pathway. This webinar will: Provide an overview of the processes involved in membrane trafficking, along with strategies to investigate them using fluorescence microscopy Offer a comprehensive guide to available labeling and detection technologies for membrane trafficking research Provide tips and tricks on how to best implement those technologies
endocytosis, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging, membrane trafficking, particles
Webinar Introduction to basic cytoskeleton labeling and detection 
The cytoskeleton is a key component of mammalian cells, providing the framework for cell migration and intracellular transport, furthermore the cytoskeleton regulates cell size and shape as well as important processes such as mitosis and endocytosis. We offer a number of solutions for researchers using fluorescent probes to study the cytoskeleton. This webinar will provide an overview of the structures that comprise the cytoskeleton and important experimental parameters. The webinar also offers a comprehensive guide to available labeling and detection technologies for cytoskeletal research as well as tips and tricks on how to best use them. These tools include those for live-cell imaging fluorescent dyes, antibodies and the BacMam gene delivery platform.
cell structure-actin, cytoskeleton, endocytosis, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins