Generation of reactive oxygen species (ROS) is inevitable for aerobic organisms and, in healthy cells, occurs at a controlled rate. Under conditions of oxidative stress, ROS production is dramatically increased, resulting in subsequent alteration of membrane lipids, proteins, and nucleic acids. Oxidative damage of these biomolecules is associated with aging as well as a variety of pathological events, including atherosclerosis, carcinogenesis, ischemia reperfusion injury, and neurodegenerative disorders.
We provide a variety of Invitrogen™ fluorescent tools to track different parameters in oxidative stress:
- Generalized oxidative stress
- Lipid peroxidation
- Selective ROS detection
- Fluorescent protein–based redox sensors
- Glutathione levels
These tools use platforms such as fluorescence microscopy, flow cytometry, or microplate analysis.