flow cytometry robotic handling and plate analyzer

Efficient. Flexible. Transformative.

The Invitrogen Attune NxT Flow Cytometer is configurable for applications benefiting from robotic integration. Customize your Attune NxT Flow Cytometer Robotics Configuration to fully leverage operating capacity, mitigate human operator error and enable rich, reproducible data.

Choose the components you need for robotic plate taxiing, extended fluidics, and temperature-stable plate storage.

Advantages of the Attune NxT Flow Cytometer Robotics Configuration include:

  • Reliable—Automated with extended unmanned runtime settings
  • Scalable—Build the Attune NxT configuration you need for your applications
  • Efficient—Maximized capacity for processing large quantities of data

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Reliable operation, innovative mechanical integrity

Multi-component flow cytometry robotics

High-throughput screening for flow cytometry requires multiple instrument components but offer autonomy to the researcher. This video shows how reliable robotic automation enables error-set-up and overnight plate screening runs using unattended mode.

Leverage the Attune NxT Flow Cytometer Robotics Configuration for outstanding performance, walkaway reliability, accurate monitoring, and productive uptime. Optimize for particular specifications, workload, and objectives of multi-instrument, high-volume labs with custom upgrades.

  • Rich data—this degree of automation enables measurably more consistency
  • Coordinated operations—boost efficiency and trim production cycle intervals
  • Sustained reliability—mechanically engineered to achieve long-lasting high-performance.

True walk-away automation in biotech and biopharma labs is not achieved solely with the integration of robotics and defined points of user intervention but rather when every instrument in the workcell is reliable. A system’s speed is inconsequential if the system is not also robust in its operation. Choose expertly engineered instruments for your automation workcell.

The components comprising the Attune NxT Flow Cytometer Robotics Configuration include:

  • Flow cytometer—speed and mechanical integrity
  • Plate sampler—consistent plate sampling
  • Expanded fluidics—high-volume fluidics for continuous run time
  • Robotic arm—precise microplate taxiing
  • Environmental Controls and microplate storage—sequential access stacks, programmed-access hotels, and available inventory management system

Attune NxT Flow Cytometer Robotics Configuration

Attune NxT robotics configuration

Watch a video of the Attune NxT Flow Cytometer configured with robotic integration. Learn about the performance, engineering, safety, and software design of the Orbitor RS Microplate Mover.

The Attune NxT Flow Cytometer automation configurability can flex to your needs, sample volume, and sample type.

Attune NxT Flow Cytometer

Attune NxT Flow Cytometer

  • Efficient—up to 10x faster than traditional flow cytometric analyzers
  • Flexible—up to 14 colors and up to 6 channels with the violet laser
  • Transformative—run difficult samples with a system that has active mechanical resistance to clogging
Attune NxT Autosampler

Attune NxT
Autosampler

  • Low carryover—standard mode <0.5% (two wash cycles); multiple-rinse capability for ultralow carryover
  • Thorough sample mixing—each well is mixed via full aspiration (no shaking) to preserve cell viability
  • Maximized sampling—small dead volume of 30 μL for 12.5–200 μL/min, 50 μL for 50–1,000 μL/min
Orbitor RS Microplate Mover

Orbitor RS Microplate Mover

  • Safe—active and passive protective features
  • Scalable—scheduling software for multiuser labs
  • Reliable—a history of excellence in automation
Momentum Integration Software

Momentum Integration Software

  • Proven performance—instrument compatibility drivers available for over 200 instruments
  • Straightforward dashboard—actively prioritize and reprioritize runs, visualize progress, and trace plates
  • Dynamic scheduling—create multiple workflows
SmartStor Microplate Inventory System

SmartStor Microplate Inventory System

  • Temperature stability—operating range between 4 to 40°C
  • Spacious inventory—capacity for 20 standard microplates or 9 deep-well blocks
  • Internal monitoring—inventory updates via internal scanning system
Attune NxT External Fluid Supply (EFS)

Attune NxT External Fluid Supply (EFS)

  • Extended fluid options—a 10 L cube increases the period of continual operation by reducing the frequency of replenishing and emptying the fluid
  • Active monitoring—waste bottle features a vent, sensor, and fill-line alarm
  • Benchtop flexibility—umbilical cords enables stowage either under-the-bench or on an adjacent cart

Table 1. Five configuration options for the Attune NxT Flow Cytometer. Configurations listed with the associated components.

Attune NxT Flow Cytometer configuration option Components
Base Configuration
  • Attune NxT Flow Cytometer (1-4 lasers)
Automation Configuration
  • Attune NxT Flow Cytometer
  • Attune Autosampler (AAS)
Automation Configuration
with Extended Fluidics
  • Attune NxT Flow Cytometer
  • Attune Autosampler
  • Attune NxT External Fluid Supply (EFS)
Robotics Configuration
  • Attune NxT Flow Cytometer
  • Attune Autosampler
  • Attune NxT External Fluid Supply (EFS)
  • Thermo Scientific Orbitor RS Microplate Mover
  • Thermo Scientific Momentum Scheduling Software
Robotics Configuration
with Sample Management
  • Attune NxT Flow Cytometer
  • Attune Autosampler
  • Attune NxT External Fluid Supply (EFS)
  • Thermo Scientific Orbitor RS Microplate Mover
  • Thermo Scientific Momentum Scheduling Software
  • Thermo Scientific SmartStor Sample Management System

Request a quote

Technical specifications

Thermo Scientific Orbitor RS 2 Microplate Mover

Thermo Scientific Orbitor RS 2 Microplate Mover

Axis/Joint
  • (Height): 575 mm (Range); 750 mm/sec (Velocity); 2250 mm/sec2 (Acceleration)
  • Radius (Reach): ±406 mm (Range); 200 mm/sec (Velocity); 3600 mm/sec2 (Acceleration)
  • Theta (Rotation): Infinite (Range); 225 deg/sec (Velocity); 675 deg/sec2 (Acceleration)
Grip
  • Plate type:  Handles most SBS conforming plates
  • Maximum microplate weight: 250 g including sample
  • Orientation:  Landscape or Portrait
  • Lids: Placement and removal
  • Plate detection: Yes
  • Barcode reading: Integrated reader in grip; compatible with freestanding reader
  • Force and speed: User-adjustable
  • Fingers: Variable plate/container designs
Operation
  • Unattended Mode: Yes, with text and email error and completion notification settings
  • Overnight Operation settings: Yes
  • Error Event Escalation settings: User-definedignore, warning, or error
  • Admin settings: Assignable roles (Administrator, Operator, Expert)
  • Computers required: (A) Co-located on one unit with Momentum software executing local control of Attune NxT software; or (B) Installed on two separate computers with Momentum software executing remote control of Attune NxT software
  • Regulatory: Research Use Only
Safety
  • Collision Detection and Recovery: Self-homing mover
  • Manual Override: Hardwired emergency stop button
Physical Characteristics
  • Power requirements: 110-230 V auto switching
  • Operating environment: 4–40°C, non-condensing
  • Safety compliances: CE, CSA
  • Weight: 25 kg (55 lb)
  • Motion Control: Closed Loop Servo, Brushless AC Motor
  • Operator control: Directly from PC, requires Windows XP or Windows Vista operating systems
Software
  • Interface format: Dashboard
  • Runtime views: Detailed
  • Mid-run sample loading/unloading: Available
  • Worklist: Automatic execution enabled; import and barcode value matching
  • Process flow: User-set time and logical constraints
  • Shared lab: Multi-user login management
  • Process validation and version control: Built in
  • Compliance: Full xml audit log CFR21-11 & CLIA compliant
  • Distributed motion: Multi-mover systems supported
  • Workflow designer: Drag and drop
  • Sample tracking: By barcodes and sample ID
  • Container definition: Built-in library
  • Simulation mode: Realtime, Accelerated
  • Progress Visualization: Plate tracing and visual progress of projects
  • Priority: Active adjustable with dynamic scheduling
Requisite Environmental Conditions
  • Maximum operating temperature:  Ambient +10° to +40°C (50° to 104°F)
  • Storage temperature: 20° to 75°C (–4° to 167°F)
  • Ambient humidity: 10% to 80% RH non-condensing
  • Occupancy: Indoor use only
Power
  • Electrical requirements: 100–240 VAC, 50/60 Hz, nominal
  • Receptacle: Requires a NEMA 5-15 female receptacle
  • Consumption: 80 W maximum
Data Management
  • Software: Thermo Scientific Momentum Laboratory Automation Workflow Software
  • Application programming interface: Invitrogen Attune NxT Software 
  • Flow cytometer: Invitrogen Attune NxT Flow Cytometer 
  • CPU: 2 GHz or faster 32-bit (X86) or 64-bit (X64)
  • RAM: 2 GB (32-bit) or 3 GB (64-bit)
  • USB ports: 2
  • License key: Yes, USB
  • Operating System: Windows 7 Professional
Elevator, Reach and Axes

Clearance (H x W x D)

  • Relative positioning: Inline or opposed configuration. Orbitor mover installs adjacent to the plate nest side of the flow cytometer
  • Overall space required for Orbitor installation: 29” x 36” x 36”
  • Without arms extended: 14”
  • With arm extended: 24”
  • With storage: 24”
Microplate Storage
  • Random access: 8 plates per hotel
  • Sequential access: 40 plates per stack
  • Maximum standard hotel capacity: 72 plates
  • Maximum standard stack capacity: 360 plates
  • Expanded capacity: Custom spines available
  • Temperature control: SmartStor storage device available
Mounting

Supporting surface area

  • Bench: Flat and sufficiently stable to support robot at 50 lbs and storage at 20 lbs minimum
  • Mounting: Required 4x M6 Machine screws or 4x #12 wood screws

  • Thermo Scientific SmartStor Microplate Storage Device

    Thermo Scientific SmartStor Microplate Storage Device

    Temperature Range
    • Controlled range: 4 to 40°C
    Inventory
    • Standard microplate: 20
    • Deep well blocks: 9
    • Internal inventory monitoring: Scanner-determined inventory updates

    Attune NxT External Fluid Supply (EFS)

    Attune NxT External Fluid Supply (EFS)

    Focusing Fluid
    • Focusing fluid container: 10 L cubetainer, single use
    • Focusing fluid connection: Cap with sensor and fluid intake
    • Umbilical 1: For focusing fluid cubetainer
    Waste
    • Umbilical 2: 6 ft connection
    • Maximum waste volume: up to 18 L (including 2 L of bleach for biosafety requirements)
    • Containment: 20 L reusable Nalgene standard container (non-autoclavable) with modified lid containing vent and sensor.
    • Full notification: Yes, alarm
    Physical Characteristics
    • H/D/W counter footprint: 10 in (25.4 cm) x 16.5 in (41.9 cm) x 25 in (63.5 cm)
    • H/D/W with containers: 22 in (55.9 cm) x 16.5 in (41.9 cm) x 25 in (63.5 cm)
    • Dry weight: Approximately 30 lb/13.6 kg
    • Weight with 10 L fluid: Approximately 62 lb/28.1 kg
    • Electrical input: From Attune NxT flow cytometer
    Operating Range
    • Operating range: 15°–32°C
    • Humidity range: 10–90% non-condensing

    Engineering of the Orbitor RS 2 Microplate Mover

    Robotics engineering for flow cytometry workcell

    Design thinking behind robotic arm for pharma screening in high-throughput environment. Why the robot is engineered with an integrated barcode reader, bi-directional wrist, and such broad plate type compatibility.

    Barcode reading

    For sample traceability, plate data storage, and barcode transmission, the Orbitor RS 2 mover is designed to synchronize barcodes. The Orbitor RS 2 mover has an on-board barcode reader in the grip (Figure 1).

    Grip
    • Grip force settings based on plate type and weight with sample
    • Grip detection
    • Integrated plate sensor
    • Bi-directional wrist for portrait or landscape clutching
    Diverse plate compatibility
    • A pre-populated list of plate types
    • Additional plate types may be added for a variety of applications
    • Lidded plates to and from storage, and un-lid and re-lid plates
    Hotels and stacks
    • Storage platforms configured with an arrangement of plates and hotels (Figure 2)
    • Random-access microplate hotels
    • Sequential-access storage stacks
    • Landscape and portrait plate orientation
    mode of action of orbitor RS plate gripper

    Figure 1. Plate gripper with integrated barcode reader. 

    storage platforms for the orbitor RS

    Figure 2. Two hotels and two stacks positioned to store and receive well plates. Extended platforms are available for expanded plate capacity.

    Straightforward, feature-rich software

    Momentum software for flow cytometry automation

    Scheduling software for robotic integration for Attune NxT Robotics Configuration automation. How the user defines the application, schedules the screening assay, and prioritizes the plate in a high-throughput lab.

    The robotic automation system is managed centrally through Thermo Scientific Momentum Laboratory Automation Workflow Software (Figure 3). Define, execute, and monitor complex processes by the Orbitor RS 2 and Attune NxT flow cytometer in a straightforward, visual environment with many user-defined and custom features.

    Momentum Scheduling software contributes to the Attune NxT Robotics Configuration with:

    • Proven performance—instrument compatibility drivers available for over 200 instruments
    • Straightforward dashboard—actively prioritize and reprioritize runs, visualize progress, and trace plates
    • Dynamic scheduling—create multiple workflows
    Home screen user interface

    Figure 3. Home screen user interface of the Thermo Scientific Momentum Laboratory Automation Workflow Software.

    The Attune NxT software is designed for compatibility with Momentum software (Figure 4).  Automation buttons on the ribbon in Attune NxT software control automation settings. When automation is enabled, Momentum software connects the Orbitor RS 2 to the Attune NxT Flow Cytometer.

    home screen and automation icons of momentum software

    Figure 4. (A) Home screen of the user-interface of the Attune NxT software. (B) Automation icons on the ribbon of the Attune NxT software.

    Designed with multiple safety features

    Lab robotics safety considerations

    This video overviews the active and passive safety technology features expected within the robotic flow cytometry automation workflow. Pharma and biotech labs conducting plate screening require split-second contact response time.

    The Attune NxT Robotics Configuration helps enable a safe working environment. The Orbitor RS 2 robot was designed with active and passive safety technology features including:

    • Rubber grip bumper—an overhead grip with protective layer against jabbing or protruding fingers
    • Self-homing mover—the mover will home itself and open up communication with instrument upon system start-up
    • Advanced error handling—Unattended Mode increase operator autonomy during extended unattended operation. This mode has "in-advance" error set-up with user-defined escalation indicated by system error beacon (Figure 5). Set email and text notifications to introduce autonomy in your routine and peace of mind during overnight runs.
    • Rapid response—the instrument responds with split-second reaction time upon minimal contact
    • Manual override mechanism—emergency stop button (Figure 6) manually halts operation; user-initiated stops and last known location logged in the system
    Momentum software for automation

    Figure 5. System error beacon for advanced error notification with “ignore” “warning” or “error” event distinctions.

    emergency stop device of the orbitor RS

    Figure 6. Emergency stop device hardwired into the Orbitor RS 2 to enable manual operation override. Standard electronic override stopping button ceases all robotic motion is split-second when pressed. 

    Experienced global Service & Support

    Invitrogen Flow Cytometry Service and Support

    What to expect for aftermarket care for integrated robotic automation for flow cytometry. This video shows the multiple facets of post-purchase service and support including Field Service Engineering, Tech Assist, post-install Technical Training.

    The Invitrogen Flow Cytometry team understands the post-purchase needs of researchers for operation and maintenance, after-market technical service, and ownership of instruments. We work with researchers every day from all over the globe. Are you new to being the point person for the flow cytometer? What is the most difficult sample you need to analyze?  Are you prepared to grow this technology within your organization? Invitrogen and Thermo Scientific instruments are supported by an extensive, experienced global team offering solutions to ensure that your Attune NxT Flow Cytometer configuration is optimized for accuracy, productivity, and performance.

    Our premier service and support network options include:

    Integration and Relocation services

    Our Field Service Engineers are available for setting up or relocating a multicomponent workcell or adding additional instruments to the configuration.  

    Service and Support

    Field Service Engineers dispatch to service calls and manage all instrument installs. An extended service option also available. Preventative maintenance is another valuable aspect of your investment.

    Technical Support

    A tech support line is available with rolling time zone routing. Speak with an engineer or technician on the phone to answer questions and troubleshoot.

    Software application support

    Take advantage of the nimble adaptability of Momentum Laboratory Automation Workflow Software to configure operational settings that help enable your automated workcell to meet your needs. Our technical specialists are able to collaborate with you to establish and modify software applications and upgrades. 

    Application specialists

    Globally, dozens of PhD scientists build the ranks of our teams for supporting, developing and partnering with you to expand the complexity of your cell-based assays, determine the best instrumentation configuration for your applications and help you just get to the science.

    Training workshops

    Post-install, labs receive a 4-hour familiarization workshop to learn about operating the Attune NxT flow cytometer in concert with the Orbitor RS 2 Microplate Mover, the External Fluid Supply and the SmartStor microplate storage device. Supplementary sessions are available for expanded operation and application optimization.

    Reliable operation, innovative mechanical integrity

    Multi-component flow cytometry robotics

    High-throughput screening for flow cytometry requires multiple instrument components but offer autonomy to the researcher. This video shows how reliable robotic automation enables error-set-up and overnight plate screening runs using unattended mode.

    Leverage the Attune NxT Flow Cytometer Robotics Configuration for outstanding performance, walkaway reliability, accurate monitoring, and productive uptime. Optimize for particular specifications, workload, and objectives of multi-instrument, high-volume labs with custom upgrades.

    • Rich data—this degree of automation enables measurably more consistency
    • Coordinated operations—boost efficiency and trim production cycle intervals
    • Sustained reliability—mechanically engineered to achieve long-lasting high-performance.

    True walk-away automation in biotech and biopharma labs is not achieved solely with the integration of robotics and defined points of user intervention but rather when every instrument in the workcell is reliable. A system’s speed is inconsequential if the system is not also robust in its operation. Choose expertly engineered instruments for your automation workcell.

    The components comprising the Attune NxT Flow Cytometer Robotics Configuration include:

    • Flow cytometer—speed and mechanical integrity
    • Plate sampler—consistent plate sampling
    • Expanded fluidics—high-volume fluidics for continuous run time
    • Robotic arm—precise microplate taxiing
    • Environmental Controls and microplate storage—sequential access stacks, programmed-access hotels, and available inventory management system

    Attune NxT Flow Cytometer Robotics Configuration

    Attune NxT robotics configuration

    Watch a video of the Attune NxT Flow Cytometer configured with robotic integration. Learn about the performance, engineering, safety, and software design of the Orbitor RS Microplate Mover.

    The Attune NxT Flow Cytometer automation configurability can flex to your needs, sample volume, and sample type.

    Attune NxT Flow Cytometer

    Attune NxT Flow Cytometer

    • Efficient—up to 10x faster than traditional flow cytometric analyzers
    • Flexible—up to 14 colors and up to 6 channels with the violet laser
    • Transformative—run difficult samples with a system that has active mechanical resistance to clogging
    Attune NxT Autosampler

    Attune NxT
    Autosampler

    • Low carryover—standard mode <0.5% (two wash cycles); multiple-rinse capability for ultralow carryover
    • Thorough sample mixing—each well is mixed via full aspiration (no shaking) to preserve cell viability
    • Maximized sampling—small dead volume of 30 μL for 12.5–200 μL/min, 50 μL for 50–1,000 μL/min
    Orbitor RS Microplate Mover

    Orbitor RS Microplate Mover

    • Safe—active and passive protective features
    • Scalable—scheduling software for multiuser labs
    • Reliable—a history of excellence in automation
    Momentum Integration Software

    Momentum Integration Software

    • Proven performance—instrument compatibility drivers available for over 200 instruments
    • Straightforward dashboard—actively prioritize and reprioritize runs, visualize progress, and trace plates
    • Dynamic scheduling—create multiple workflows
    SmartStor Microplate Inventory System

    SmartStor Microplate Inventory System

    • Temperature stability—operating range between 4 to 40°C
    • Spacious inventory—capacity for 20 standard microplates or 9 deep-well blocks
    • Internal monitoring—inventory updates via internal scanning system
    Attune NxT External Fluid Supply (EFS)

    Attune NxT External Fluid Supply (EFS)

    • Extended fluid options—a 10 L cube increases the period of continual operation by reducing the frequency of replenishing and emptying the fluid
    • Active monitoring—waste bottle features a vent, sensor, and fill-line alarm
    • Benchtop flexibility—umbilical cords enables stowage either under-the-bench or on an adjacent cart

    Table 1. Five configuration options for the Attune NxT Flow Cytometer. Configurations listed with the associated components.

    Attune NxT Flow Cytometer configuration option Components
    Base Configuration
    • Attune NxT Flow Cytometer (1-4 lasers)
    Automation Configuration
    • Attune NxT Flow Cytometer
    • Attune Autosampler (AAS)
    Automation Configuration
    with Extended Fluidics
    • Attune NxT Flow Cytometer
    • Attune Autosampler
    • Attune NxT External Fluid Supply (EFS)
    Robotics Configuration
    • Attune NxT Flow Cytometer
    • Attune Autosampler
    • Attune NxT External Fluid Supply (EFS)
    • Thermo Scientific Orbitor RS Microplate Mover
    • Thermo Scientific Momentum Scheduling Software
    Robotics Configuration
    with Sample Management
    • Attune NxT Flow Cytometer
    • Attune Autosampler
    • Attune NxT External Fluid Supply (EFS)
    • Thermo Scientific Orbitor RS Microplate Mover
    • Thermo Scientific Momentum Scheduling Software
    • Thermo Scientific SmartStor Sample Management System

    Request a quote

    Technical specifications

    Thermo Scientific Orbitor RS 2 Microplate Mover

    Thermo Scientific Orbitor RS 2 Microplate Mover

    Axis/Joint
    • (Height): 575 mm (Range); 750 mm/sec (Velocity); 2250 mm/sec2 (Acceleration)
    • Radius (Reach): ±406 mm (Range); 200 mm/sec (Velocity); 3600 mm/sec2 (Acceleration)
    • Theta (Rotation): Infinite (Range); 225 deg/sec (Velocity); 675 deg/sec2 (Acceleration)
    Grip
    • Plate type:  Handles most SBS conforming plates
    • Maximum microplate weight: 250 g including sample
    • Orientation:  Landscape or Portrait
    • Lids: Placement and removal
    • Plate detection: Yes
    • Barcode reading: Integrated reader in grip; compatible with freestanding reader
    • Force and speed: User-adjustable
    • Fingers: Variable plate/container designs
    Operation
    • Unattended Mode: Yes, with text and email error and completion notification settings
    • Overnight Operation settings: Yes
    • Error Event Escalation settings: User-definedignore, warning, or error
    • Admin settings: Assignable roles (Administrator, Operator, Expert)
    • Computers required: (A) Co-located on one unit with Momentum software executing local control of Attune NxT software; or (B) Installed on two separate computers with Momentum software executing remote control of Attune NxT software
    • Regulatory: Research Use Only
    Safety
    • Collision Detection and Recovery: Self-homing mover
    • Manual Override: Hardwired emergency stop button
    Physical Characteristics
    • Power requirements: 110-230 V auto switching
    • Operating environment: 4–40°C, non-condensing
    • Safety compliances: CE, CSA
    • Weight: 25 kg (55 lb)
    • Motion Control: Closed Loop Servo, Brushless AC Motor
    • Operator control: Directly from PC, requires Windows XP or Windows Vista operating systems
    Software
    • Interface format: Dashboard
    • Runtime views: Detailed
    • Mid-run sample loading/unloading: Available
    • Worklist: Automatic execution enabled; import and barcode value matching
    • Process flow: User-set time and logical constraints
    • Shared lab: Multi-user login management
    • Process validation and version control: Built in
    • Compliance: Full xml audit log CFR21-11 & CLIA compliant
    • Distributed motion: Multi-mover systems supported
    • Workflow designer: Drag and drop
    • Sample tracking: By barcodes and sample ID
    • Container definition: Built-in library
    • Simulation mode: Realtime, Accelerated
    • Progress Visualization: Plate tracing and visual progress of projects
    • Priority: Active adjustable with dynamic scheduling
    Requisite Environmental Conditions
    • Maximum operating temperature:  Ambient +10° to +40°C (50° to 104°F)
    • Storage temperature: 20° to 75°C (–4° to 167°F)
    • Ambient humidity: 10% to 80% RH non-condensing
    • Occupancy: Indoor use only
    Power
    • Electrical requirements: 100–240 VAC, 50/60 Hz, nominal
    • Receptacle: Requires a NEMA 5-15 female receptacle
    • Consumption: 80 W maximum
    Data Management
    • Software: Thermo Scientific Momentum Laboratory Automation Workflow Software
    • Application programming interface: Invitrogen Attune NxT Software 
    • Flow cytometer: Invitrogen Attune NxT Flow Cytometer 
    • CPU: 2 GHz or faster 32-bit (X86) or 64-bit (X64)
    • RAM: 2 GB (32-bit) or 3 GB (64-bit)
    • USB ports: 2
    • License key: Yes, USB
    • Operating System: Windows 7 Professional
    Elevator, Reach and Axes

    Clearance (H x W x D)

    • Relative positioning: Inline or opposed configuration. Orbitor mover installs adjacent to the plate nest side of the flow cytometer
    • Overall space required for Orbitor installation: 29” x 36” x 36”
    • Without arms extended: 14”
    • With arm extended: 24”
    • With storage: 24”
    Microplate Storage
    • Random access: 8 plates per hotel
    • Sequential access: 40 plates per stack
    • Maximum standard hotel capacity: 72 plates
    • Maximum standard stack capacity: 360 plates
    • Expanded capacity: Custom spines available
    • Temperature control: SmartStor storage device available
    Mounting

    Supporting surface area

  • Bench: Flat and sufficiently stable to support robot at 50 lbs and storage at 20 lbs minimum
  • Mounting: Required 4x M6 Machine screws or 4x #12 wood screws

  • Thermo Scientific SmartStor Microplate Storage Device

    Thermo Scientific SmartStor Microplate Storage Device

    Temperature Range
    • Controlled range: 4 to 40°C
    Inventory
    • Standard microplate: 20
    • Deep well blocks: 9
    • Internal inventory monitoring: Scanner-determined inventory updates

    Attune NxT External Fluid Supply (EFS)

    Attune NxT External Fluid Supply (EFS)

    Focusing Fluid
    • Focusing fluid container: 10 L cubetainer, single use
    • Focusing fluid connection: Cap with sensor and fluid intake
    • Umbilical 1: For focusing fluid cubetainer
    Waste
    • Umbilical 2: 6 ft connection
    • Maximum waste volume: up to 18 L (including 2 L of bleach for biosafety requirements)
    • Containment: 20 L reusable Nalgene standard container (non-autoclavable) with modified lid containing vent and sensor.
    • Full notification: Yes, alarm
    Physical Characteristics
    • H/D/W counter footprint: 10 in (25.4 cm) x 16.5 in (41.9 cm) x 25 in (63.5 cm)
    • H/D/W with containers: 22 in (55.9 cm) x 16.5 in (41.9 cm) x 25 in (63.5 cm)
    • Dry weight: Approximately 30 lb/13.6 kg
    • Weight with 10 L fluid: Approximately 62 lb/28.1 kg
    • Electrical input: From Attune NxT flow cytometer
    Operating Range
    • Operating range: 15°–32°C
    • Humidity range: 10–90% non-condensing

    Engineering of the Orbitor RS 2 Microplate Mover

    Robotics engineering for flow cytometry workcell

    Design thinking behind robotic arm for pharma screening in high-throughput environment. Why the robot is engineered with an integrated barcode reader, bi-directional wrist, and such broad plate type compatibility.

    Barcode reading

    For sample traceability, plate data storage, and barcode transmission, the Orbitor RS 2 mover is designed to synchronize barcodes. The Orbitor RS 2 mover has an on-board barcode reader in the grip (Figure 1).

    Grip
    • Grip force settings based on plate type and weight with sample
    • Grip detection
    • Integrated plate sensor
    • Bi-directional wrist for portrait or landscape clutching
    Diverse plate compatibility
    • A pre-populated list of plate types
    • Additional plate types may be added for a variety of applications
    • Lidded plates to and from storage, and un-lid and re-lid plates
    Hotels and stacks
    • Storage platforms configured with an arrangement of plates and hotels (Figure 2)
    • Random-access microplate hotels
    • Sequential-access storage stacks
    • Landscape and portrait plate orientation
    mode of action of orbitor RS plate gripper

    Figure 1. Plate gripper with integrated barcode reader. 

    storage platforms for the orbitor RS

    Figure 2. Two hotels and two stacks positioned to store and receive well plates. Extended platforms are available for expanded plate capacity.

    Straightforward, feature-rich software

    Momentum software for flow cytometry automation

    Scheduling software for robotic integration for Attune NxT Robotics Configuration automation. How the user defines the application, schedules the screening assay, and prioritizes the plate in a high-throughput lab.

    The robotic automation system is managed centrally through Thermo Scientific Momentum Laboratory Automation Workflow Software (Figure 3). Define, execute, and monitor complex processes by the Orbitor RS 2 and Attune NxT flow cytometer in a straightforward, visual environment with many user-defined and custom features.

    Momentum Scheduling software contributes to the Attune NxT Robotics Configuration with:

    • Proven performance—instrument compatibility drivers available for over 200 instruments
    • Straightforward dashboard—actively prioritize and reprioritize runs, visualize progress, and trace plates
    • Dynamic scheduling—create multiple workflows
    Home screen user interface

    Figure 3. Home screen user interface of the Thermo Scientific Momentum Laboratory Automation Workflow Software.

    The Attune NxT software is designed for compatibility with Momentum software (Figure 4).  Automation buttons on the ribbon in Attune NxT software control automation settings. When automation is enabled, Momentum software connects the Orbitor RS 2 to the Attune NxT Flow Cytometer.

    home screen and automation icons of momentum software

    Figure 4. (A) Home screen of the user-interface of the Attune NxT software. (B) Automation icons on the ribbon of the Attune NxT software.

    Designed with multiple safety features

    Lab robotics safety considerations

    This video overviews the active and passive safety technology features expected within the robotic flow cytometry automation workflow. Pharma and biotech labs conducting plate screening require split-second contact response time.

    The Attune NxT Robotics Configuration helps enable a safe working environment. The Orbitor RS 2 robot was designed with active and passive safety technology features including:

    • Rubber grip bumper—an overhead grip with protective layer against jabbing or protruding fingers
    • Self-homing mover—the mover will home itself and open up communication with instrument upon system start-up
    • Advanced error handling—Unattended Mode increase operator autonomy during extended unattended operation. This mode has "in-advance" error set-up with user-defined escalation indicated by system error beacon (Figure 5). Set email and text notifications to introduce autonomy in your routine and peace of mind during overnight runs.
    • Rapid response—the instrument responds with split-second reaction time upon minimal contact
    • Manual override mechanism—emergency stop button (Figure 6) manually halts operation; user-initiated stops and last known location logged in the system
    Momentum software for automation

    Figure 5. System error beacon for advanced error notification with “ignore” “warning” or “error” event distinctions.

    emergency stop device of the orbitor RS

    Figure 6. Emergency stop device hardwired into the Orbitor RS 2 to enable manual operation override. Standard electronic override stopping button ceases all robotic motion is split-second when pressed. 

    Experienced global Service & Support

    Invitrogen Flow Cytometry Service and Support

    What to expect for aftermarket care for integrated robotic automation for flow cytometry. This video shows the multiple facets of post-purchase service and support including Field Service Engineering, Tech Assist, post-install Technical Training.

    The Invitrogen Flow Cytometry team understands the post-purchase needs of researchers for operation and maintenance, after-market technical service, and ownership of instruments. We work with researchers every day from all over the globe. Are you new to being the point person for the flow cytometer? What is the most difficult sample you need to analyze?  Are you prepared to grow this technology within your organization? Invitrogen and Thermo Scientific instruments are supported by an extensive, experienced global team offering solutions to ensure that your Attune NxT Flow Cytometer configuration is optimized for accuracy, productivity, and performance.

    Our premier service and support network options include:

    Integration and Relocation services

    Our Field Service Engineers are available for setting up or relocating a multicomponent workcell or adding additional instruments to the configuration.  

    Service and Support

    Field Service Engineers dispatch to service calls and manage all instrument installs. An extended service option also available. Preventative maintenance is another valuable aspect of your investment.

    Technical Support

    A tech support line is available with rolling time zone routing. Speak with an engineer or technician on the phone to answer questions and troubleshoot.

    Software application support

    Take advantage of the nimble adaptability of Momentum Laboratory Automation Workflow Software to configure operational settings that help enable your automated workcell to meet your needs. Our technical specialists are able to collaborate with you to establish and modify software applications and upgrades. 

    Application specialists

    Globally, dozens of PhD scientists build the ranks of our teams for supporting, developing and partnering with you to expand the complexity of your cell-based assays, determine the best instrumentation configuration for your applications and help you just get to the science.

    Training workshops

    Post-install, labs receive a 4-hour familiarization workshop to learn about operating the Attune NxT flow cytometer in concert with the Orbitor RS 2 Microplate Mover, the External Fluid Supply and the SmartStor microplate storage device. Supplementary sessions are available for expanded operation and application optimization.

    3D Demo: Explore the Attune NxT Robotics Configuration

    Flow Cytometry applications for immuno-oncology research

    Empowering technologies for immuno-oncology research—Accelerating discovery with simplified workflows for flow cytometry, biomarker profiling, and cell imaging.
    Get the guide

    Find solutions in the Flow Capabilities Guide

    With instruments, reagents, antibodies, and buffers, the Invitrogen Flow Cytometry portfolio is comprehensively addressing diverse workflows.
    Get the guide

    Design your flow panels using the Invitrogen Flow Cytometer Panel Builder with advanced functionality.
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    Learn what to consider when compare flow cytometers made by multiple manufacturers.
    thermofisher.com/compareflow

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