stacked histograms showing emission of compensation beads

Compensation simplified

Invitrogen OneComp and UltraComp eBeads compensation particles are polystyrene microspheres that contain a mixture of antibody-coated positive compensation beads and uncoated negative compensation beads, combined in one vial. Dispense the eBeads as a single drop for compensation made easy. UltraComp eBeads microspheres were developed with the same benefits as OneComp eBeads microspheres with the added advantage of being optimized for use on the violet laser. eBeads cross-react to some antibodies of rabbit origin, but have not been extensively tested for this reactivity.

UltraComp eBeads microspheres

Traditional compensation beads, when used on the violet laser, typically provide poor compensation values when incorporating the increasing number of violet-emitting fluorochromes. UltraComp eBeads were developed to provide a reliably bright signal, resulting in greater accuracy when calculating spillover values.

Advantages of UltraComp eBeads microspheres:

  • Optimized for the violet laser
  • Supplied in a single vial—one drop contains both positive and negative beads
  • Suitable for multiple species: hamster, mouse, rat
  • Recognizes both kappa and lambda chains
stacked histograms showing emission for UltraComp

Figure 1. Staining of UltraComp eBeads with 13 different Invitrogen eFluor 450 dye–conjugated monoclonal antibodies, including one of each subclass commonly used in flow cytometry. Beads were stained with 0.25 µg of each antibody and analyzed by flow cytometry. Each histogram represents one staining antibody (isotype and marker indicated at right, except for Rat IgG2a and Hamster antibodies, for which the isotype and clone are indicated).

scatter plots showing compensation using UltraComp eBeads

Figure 2. Using UltraComp eBeads microspheres for compensation of Invitrogen eFluor 506 dye–conjugated antibodies results in accurately compensated data.

Comparison of UltraComp and OneComp eBeads
  UltraComp eBeads OneComp eBeads
Reactivity Hamster, mouse, and rat antibodies and recognition of the kappa & lambda chains
Format One vial, dispense as a single drop
Laser compatibility Compatible with most standard lasers, UV to 633 nm Not compatible with violet lasers
Size 25 tests 100 tests 25 tests 100 tests
Cat. No. 01-2222-41 01-2222-42 01-1111-41 01-1111-42

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