Figure 1. Analysis of carryover from a tube. (A) A forward scatter (FSC) vs. side scatter (SSC) plot of human lysed whole blood at 106 cells/mL, and (B) analysis of PBS blank with the same gates. The numbers displayed for each population of lymphocytes, monocytes, and granulocytes are the concentrations in cells/µL. (C) Percent carryover of lysed whole blood (LWB) as a function of cell concentration for the three main cell population gates; 50 µL of lysed whole blood was acquired, followed by 50 µL of PBS. Percent carryover = (concentration of PBS subset/concentration of LWB subset) x 100%. In this example, the lymphocyte carryover is (21/12,702) x 100% = 0.17%. The error bars show the standard deviation (n = 3) of the calculated percent carryover at each concentration.
The percent carryover of each of individual subset is calculated as:
Percent carryover = PBS subset/LWB subset x 100
In example shown in figures 1A and 1B, the lymphocyte carryover is calculated at 21/12,702 x 100 = 0.17%