Invitrogen eBioscience Super Bright 436 dye is a polymer-based fluorophore excited by the violet laser (405 nm) that emits signal at 436 nm with high efficacy. The technology behind the Super Bright polymer dye family makes them useful for identifying low-abundance markers on a cell surface in flow cytometry.

An alternative to Brilliant Violet 421 conjugates, antibody conjugates of Super Bright 436 provide increased resolution of positive and negative populations, but with less background and less interfering dye–dye interaction than Brilliant Violet 421 conjugates. In addition, antibody conjugates of this polymer dye are significantly brighter than Invitrogen eFluor 450 conjugates and Pacific Blue conjugates.

Executing a well-designed experiment requires multiple reagents. Super Bright conjugates are fully compatible with Invitrogen UltraComp eBeads microspheres for compensation, viability stains, fluorescent dyes and other reagents commonly used in flow cytometry. Robust, highly sensitive, and stable, Super Bright conjugates easily fit into any flow cytometry panel.

We offer Super Bright 436 dye conjugated to primary antibodies for use in flow cytometry. 

Super Bright 436 dye dashboard


Initial brightness

 
 
 
 
 
The violet laser–excitable Super Bright 436 dye is optimized for use in flow cytometry applications.  
   

Photostability in buffer

 
 
 
 
 

  405 450/50 414 436  
 
Laser line

Common filter set

Excitation max

Emission max
 

Super Bright 436 dye products

We offer Super Bright 436 dye conjugated to primary antibodies for use in flow cytometry.

Learn more here:

Primary antibodies for the violet laser

Invitrogen eBioscience Super Bright polymer dyes are violet laser–excitable fluorophores that expand the capabilities of multi-parametric flow cytometry analysis. This family of bright fluorophores enables the use of more markers off the violet channel and increases the detection of low-abundance antigens due to their super bright signals.

The Super Bright polymer dyes join the trusted Invitrogen Alexa Fluor dye and Invitrogen eFluor dye portfolios in providing fluorochromes compatible with the 405 nm spectral line of the violet laser.

Resources

Primary antibodies to match your cytometer's lasers

To support your multicolor flow cytometry experiments, our antibody portfolios include a variety of dyes with compatibility to all commonly used flow cytometry lasers.

Fluorescence SpectraViewer

Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

Go to the Fluorescence SpectraViewer