Gibco WFI—Certified for Confidence

Quality WFI for cell culture for all your upstream and downstream bioprocessing needs

Gibco™ Water for Injection (WFI) is pure, cell culture–grade water that enables consistent and reproducible results.

Our cell culture–grade water means it meets the highest of standards in accordance with the biopharmaceutical, pharmaceutical, and diagnostic industries. Every batch undergoes strict quality control testing and utilizes our vapor compression distillation system.

Gibco WFI for cell culture meets high quality control standards:

  • Complies with both United States Pharmacopeia(USP) and European Pharmacopoeia (EP) specifications
  • Manufactured in ISO–certified, cGMP, and FDA–registered facilities
  • Membrane–filtered to 0.1 µm
  • 24–month shelf life
  • Extended storage temperature of 2–30°C

 

To order new WFI for cell culture catalog offerings in 20 L and 200 L sizes, please call our customer care team at 1-800-955-6288 or email us at customerservice@thermofisher.com for orders in the United States.

Our purification process produces WFI for cell culture of high quality, prepared by distillation and filtration (0.1 µm), with no added substances. Water quality is monitored on a routine basis; conductivity, total organic carbon, endotoxins, and bioburden are tested daily; metals are tested monthly.

Get pure, cell culture-grade water in a variety of custom sizes to suit your bioprocessing needs.


Gibco WFI for cell culture specifications:

Gibco WFI for cell culture meets the USP/EP monograph for water for injection at point of fill, packaged in bulk for commercial use elsewhere.

The Certificate of Analysis (CofA) provides the following parameters:

Parameter Limits Units
Conductivity ≤5.0 µS/cm
Endotoxin ≤0.25 EU/mL
Nitrate ≤0.2 ppm
Osmolality 0–20 mOsm/kg
pH 4.0–7.5  
Sterility Negative  
Total organic carbon ≤500 ppb

Sample Certificate of Analysis Report


Applications of WFI for Cell Culture

WFI for Cell Culture can be used for a wide variety of upstream and downstream process solutions.

Upstream

  • Cell culture media
  • Cell suspension and wash solutions
  • Formulating solutions
  • Sanitization of laboratory glassware and containers
  • Reconstitution of products, such as dry powder media

Downstream

  • Diluting buffers
  • Process buffers
  • Purification buffers
  • Cleaning solutions and rinse agents

Caution: For use as a raw material in further manufacturing applications.