What are isoschizomers?

Isoschizomers are restriction enzymes that have the same recognition sequence and the same specificity. The prototype enzyme is generally the first to be discovered and characterized. Subsequent enzyme(s) discovered from different hosts that recognize and cut at the same target sequence are termed “isoschizomers” of that prototype. Because enzyme naming conventions reference the genus, species, and strain from which they were isolated, isoschizomers could have very different names, despite the sequence recognition similarities between them. For example, Anza 3 BcuI is an isoschizomer of SpeI. Review the table below outlining common Anza isoschizomers offered in our Anza Restriction Enzyme Cloning System.

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Prototype Anza isoschizomer Recognition and cutting site
SpeI Anza 3 BcuI A^CTAG T
T GATC^A
AgeI Anza 7 BshTI A^CCGG T
T GGCC^A
NdeI Anza 9 NdeI CA^TA TG
GT AT^AC
AvrII Anza 15 XmaJI C^CTAG G
G GATC^C
AscI Anza 21 SgsI GG^CGCG CC
CC GCGC^GG
PmeI Anza 24 MssI
GTTT^AAAC
CAAA^TTG
SphI

Anza 25 PaeI

G CATG^C
C^GTAC G
EcoRV Anza 26 Eco32I GAT^ATC
CAN^TAG
ClaI Anza 30 Bsu15I AT^CG AT
TA GC^TA
MfeI Anza 31 MunI C^AATT G
G TTAA^C
SapI Anza 33 LguI GCTCTTCN1^
CGAGAAGN4^
DdeI Anza 41 HpyF3I C^TNA G
G ANT^C
SnaBI Anza 43 Eco105I TAC^GTA
ATG^CAT
SwaI Anza 49 SmiI ATTT^AAAT
TAAA^TTTA
HpaI Anza 50 KspAI GTT^AAC
CAA^TTG
PvuI Anza 52 PvuII CG AT^CG
GC^AT GC

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