Competent Cells for High Throughput Cloning
We have designed medium and high-throughput (HTP) configurations of chemically competent cells, to accommodate your increasing experimental needs. MultiShot Competent Cells are available in three packaging formats, StripWell, FlexPlate, and TOP10 96-well plates offering you flexibility in the number of transformations that can be performed at once for cloning. Choose from one of our convenient product designs.
Which Invitrogen MultiShot product is right for you?
|StripWell Chemically Competent E. Coli
Competent E. Coli
|TOP10 Chemically Competent E. Coli
|Format||12 x 8-tube strips||96-well PCR plate that can be separated into
12 x 8-well segments
|1 rack (12 x 8-tube strips)||1 segmented plate||5 plates|
Volume per well
|50 μL||20 μL||15 μL|
Transformation Efficiencies of DH5α Competent E. coli and DH5α derivatives
Figure 1. Comparing DH5α competent E. coli and 5α derivatives in multiple product configurations. Transformation efficiency was measured by performing triplicate transformations, using 10 pg pUC19 DNA, per manufacturer’s recommended protocol. Each transformation was plated in duplicate.
Find the MultiShot strain and format that you need
We also offer many other different strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options contact us for custom formatting options.
Find tips, troubleshooting help, and resources for your
Competent cells resources
Have any questions on competent cells or transformation? Click below to access overviews, videos, genotype guides and educational resources.
For Research Use Only. Not for use in diagnostic procedures.
Which Competent Cell Strain is Right for You?
|96-well plate format for HTP cloning||Versatile stripwell tubes for medium to HTP applications||Highest efficiency in versatile stripwell format|
|Format||96-well plates for automated applications||12 strips of 8 tubes in a 96-well plate format||12 strips of 8 tubes in a 96-well plate format|
|Flexibility||Use as needed||8 reaction combinations|
|Volume / well||15 µL||50 µL||50 µL|
|Kit size||5 plates||1 plate||1 plate|
|Transformation efficiency||>1x108 cfu/µg||>1x108 cfu/µg||>1x109 cfu/µg
|T1 and T5 Phage resistant (ton A)|
|Time to pick colonies||Overnight||Overnight||Same day|
|Time to purify plasmid DNA after inoculation||Overnight||Overnight||As soon as 4 hrs|
|mcrA for efficient transformation of methylated DNA from genomic preparations|
|Order Now||Order Now||Order Now|